Abstract
A new yeast, isolated from natural osmophilic sources, produces d-arabitol as the main metabolic product from glucose. According to 18S rRNA analysis, the NH-9 strain belongs to the genus Kodamaea. The optimal culture conditions for inducing production of d-arabitol were 37 °C, neutral pH, 220 rpm shaking, and 5% inoculum. The yeast produced 81.2 ± 0.67 g L−1 d-arabitol from 200 g L−1 d-glucose in 72 h with a yield of 0.406 g g−1 glucose and volumetric productivity \( Q_{\text{P}} \) of 1.128 g L−1 h−1. Semi-continuous repeated-batch fermentation was performed in shaker-flasks to enhance the process of d-arabitol production by Kodamaea ohmeri NH-9 from d-glucose. Under repeated-batch culture conditions, the highest volumetric productivity was 1.380 g L−1 h−1.
Similar content being viewed by others
References
Emodi A (1978) Xylitol: its properties and food applications. Food Technol 32:28–32
Peldyak J, Mäkinen KK (2002) Xylitol for caries prevention. J Dent Hyg 76:276–285
Nigam P, Singh D (1995) Processes for fermentative production of xylitol. Process Biochem 30:117–124
Ikeuchi T, Azuma M, Kato J, Ooshima H (1999) Screening of microorganisms for xylitol production and fermentation behavior in high concentrations of xylose. Biomass Bioenergy 16:333–339
Winkelhausen E, Kuzmanova S (1998) Microbial conversion of d-xylose to xylitol. J Ferment Bioeng 86:1–14
Sirisansaneeyyakul S, Staniszewski M, Rizzi M (1995) Screening of yeasts for production of xylitol from d-xylose. J Ferment Bioeng 80:565–570
Onishi H, Suzuki T (1969) Microbial production of xylitol from glucose. Appl Microbiol 18:1031–1035
Suzuki S, Sugiyama M, Mihara Y, Hashiguchi K (2002) Novel enzymatic method for the production of xylitol from D-arabitol by Gluconobacter oxydans. Biosci Biotechnol Biochem 66(12):2614–2620
Kiyomoto U (1971) Process for the production of d-arabitol. US Patent US3607652
Nozaki H, Suzuki S, Tsuyoshi N, Yokozeki K (2003) Production of d-arabitol by Metschnikowia reukaufii AJ14787. Biosci Biotechnol Biochem 67(9):1923–1929
Kurtzman CP (1998) Pichia. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 252–273
Zhou XL, Shen W, Rao ZM, Wang ZX, Zhuge J (2004) A rapid method for preparation of fungal chromosome DNA. Weishengwu Xue Tongbao 31(4):89–92
Shih IL, Shen MH (2006) Application of response surface methodology to optimize production of poly-e-lysine by Streptomyces albulus IFO 14147. Enzyme Microbial Technol 39:15–21
Yamada Y, Suzuki T, Matsuda M, Mikata K (1995) The phylogeny of Yamadazyma ohmeri (Etchells et Bell) Billon-Grand based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Kodamaea gen. nov. (Saccharomycetaceae). Biosci Biotechnol Biochem 59:1172–1174
Taj-Aldeen SJ, Doiphode SH, Han XY (2006) Kodamaea (Pichia) ohmeri fungaemia in a premature neonate. J Med Microbiol 55:237–239
Badal CS, Yoshikiyo S, Michael AC (2007) Production of D-arabitol by a newly isolated Zygosaccharomyces rouxii. J Ind Microbiol Biotechnol 34:519–523
Groleau D, Chevalier P, Yuen TH (1965) Production of polyols and ethanol by the osmophilic yeast Zygosaccharomyces rouxii. Biotechnol Lett 17:315–320
Noble FM, daCosta MS (1985) Factors favoring the accumulation of arabinitol in the yeast Debaryomyces hansenii. Can J Microbiol 31:467–471
Bernard EM, Christiansen KJ, Tsang SF, Keihn TE, Armstrong D (1981) Rate of arabinitol production by pathogenic yeast species. J Clin Microbiol 14:189–194
Onishi H, Saito T (1962) Polyalcohol production by Pichia miso in a jar fermentor. Agric Biol Chem 26:804–808
Acknowledgments
This work was supported by the National Basic Research Program of China (973) (2007CB714304), the National Nature Science Foundation of China (No. 20674038), Doctor Program of High Education (No. 20050291001) and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 08KJA180001).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhu, HY., Xu, H., Dai, XY. et al. Production of d-arabitol by a newly isolated Kodamaea ohmeri . Bioprocess Biosyst Eng 33, 565–571 (2010). https://doi.org/10.1007/s00449-009-0378-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-009-0378-x