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Optimization of fermentation conditions for production of anti-TMV extracellular ribonuclease by Bacillus cereus using response surface methodology

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Abstract

Bacillus cereus ZH14 was previously found to produce a new type of antiviral ribonuclease, which was secreted into medium and active against tobacco mosaic virus. In order to enhance the ribonuclease production, in this study the optimization of culture conditions using response surface methodology was done. The fermentation variables including culture temperature, initial pH, inoculum size, sucrose, yeast extract, MgSO4·7H2O, and KNO3 were considered for selection of significant ones by using the Plackett–Burman design, and four significant variables (sucrose, yeast extract, MgSO4·7H2O, and KNO3) were further optimized by a 24 factorial central composite design. The optimal combination of the medium constituents for maximum ribonuclease production was determined as 8.50 g/l sucrose, 9.30 g/l yeast extract, 2.00 g/l MgSO4·7H2O, and 0.62 g/l KNO3. The enzyme activity was increased by 60%. This study will be helpful to the future commercial development of the new bacteria-based antiviral ribonuclease fermentation process.

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Acknowledgments

This research was supported by the China Center of Industrial Culture Collection and the China National Research Institute of Food and Fermentation Industries.

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Authors and Affiliations

  1. Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai, 200240, China

    Wen-Wen Zhou & Jian-Jiang Zhong

  2. College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Beijing, 100083, China

    Yun-Long He & Tian-Gui Niu

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  1. Wen-Wen Zhou
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  2. Yun-Long He
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Correspondence to Wen-Wen Zhou.

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Zhou, WW., He, YL., Niu, TG. et al. Optimization of fermentation conditions for production of anti-TMV extracellular ribonuclease by Bacillus cereus using response surface methodology. Bioprocess Biosyst Eng 33, 657–663 (2010). https://doi.org/10.1007/s00449-009-0330-0

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  • DOI: https://doi.org/10.1007/s00449-009-0330-0

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