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Combined dissolved oxygen and pH control strategy to improve the fermentative production of l-isoleucine by Brevibacterium lactofermentum

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Abstract

The effect of both dissolved oxygen (DO) and pH on l-isoleucine production by batch culture of Brevibacterium lactofermentum was investigated. A two-stage agitation speed control strategy was developed, and the isoleucine production reached 23.3 g L−1 in a relative short time (52 h), increased by 11.6% compared to the results obtained in the single agitation speed control process. In order to make sure whether the combination of DO and pH control can boost the production by a mutual effect, different control modes were conducted, based on the data obtained from the two-stage agitation speed control strategy and the analysis of kinetics parameters at different pH values. The results showed that the mode of combining two-stage DO with two-stage pH control strategy was the optimal for isoleucine production. The isoleucine production can reach 26.6 g L−1 at 56 h, increased by 14.3% comparing to that obtained by the single two-stage DO control strategy.

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Acknowledgments

This project was financially supported by grants from the National High Technology Research and Development Program of China (863 Program, No. 2007AA02Z229), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0532), and the National Science Fund for Distinguished Young Scholars of China (No. 20625619).

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Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, 214122, Wuxi, China

    Zhijian Peng, Jun Fang, Jianghua Li, Long Liu, Guocheng Du & Jian Chen

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China

    Zhijian Peng, Jun Fang, Jianghua Li, Long Liu, Guocheng Du & Jian Chen

  3. State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China

    Jian Chen & Xiaoyuan Wang

  4. Jinghai Amino Acid Liability Limited Company, 214199, Wuxi, China

    Jianfei Ning & Liming Cai

Authors
  1. Zhijian Peng
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  2. Jun Fang
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  3. Jianghua Li
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  4. Long Liu
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  5. Guocheng Du
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  6. Jian Chen
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  7. Xiaoyuan Wang
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  8. Jianfei Ning
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  9. Liming Cai
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Corresponding authors

Correspondence to Jianghua Li or Jian Chen.

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Peng, Z., Fang, J., Li, J. et al. Combined dissolved oxygen and pH control strategy to improve the fermentative production of l-isoleucine by Brevibacterium lactofermentum . Bioprocess Biosyst Eng 33, 339–345 (2010). https://doi.org/10.1007/s00449-009-0329-6

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  • DOI: https://doi.org/10.1007/s00449-009-0329-6

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