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A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring

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Abstract

To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.

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Authors and Affiliations

  1. Institut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover, Callinstr. 3, 30167, Hannover, Germany

    Tim Höpfner, Arne Bluma, Guido Rudolph, Patrick Lindner & Thomas Scheper

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  1. Tim Höpfner
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  2. Arne Bluma
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  3. Guido Rudolph
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  4. Patrick Lindner
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  5. Thomas Scheper
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Correspondence to Tim Höpfner.

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Höpfner, T., Bluma, A., Rudolph, G. et al. A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring. Bioprocess Biosyst Eng 33, 247–256 (2010). https://doi.org/10.1007/s00449-009-0319-8

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  • DOI: https://doi.org/10.1007/s00449-009-0319-8

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