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Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus

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Abstract

Three different dissolved oxygen (DO) control approaches were proposed to improve hyaluronic acid (HA) production: a three-stage agitation speed control approach, a two-stage DO control approach, and an oxygen vector perfluorodecalin (PFC) applied approach. In the three-stage agitation speed control approach, agitation speed was 200 rpm during 0–8 h, 400 rpm during 8–12 h, and 600 rpm during 12–20 h. In the two-stage DO control strategy, DO was controlled at above 10% during 0–8 h and at 5% during 8–20 h. In the PFC applied approach, PFC (3% v/v) was added at 8 h. HA production reached 5.5 g/L in the three-stage agitation speed control culture model, and 6.3 g/L in two-stage DO control culture model, and 6.6 g/L in the PFC applied culture model. Compared with the other two DO control approaches, the PFC applied approach had a lower shear stress and thus a higher HA production was achieved.

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References

  1. Laurent TC, Laurent UB, Fraser JR (1996) The structure and function of hyaluronan: an overview. Immunol Cell Biol 74:1–7

    Article  Google Scholar 

  2. Peyron JG (1993) A new approach to the treatment of osteoarthritis: viscosupplementation. Osteoarthr Cartil 1:85–87

    Article  CAS  Google Scholar 

  3. Lapcik L, De Smedt S, Demeester J, Chabrecek P (1998) Hyaluronan: preparation, structure, properties, and applications. Chem Rev 98:2663–2684

    Article  CAS  Google Scholar 

  4. Morra M (2005) Engineering of biomaterials surfaces by hyaluronan. Biomacromolecules 6:1205–1223

    Article  CAS  Google Scholar 

  5. Park SN, Lee HJ, Lee KH, Suh H (2003) Biological characterization of EDC cross-linked collagen–hyaluronic acid matrix in dermal tissue restoration. Biomaterials 24:1631–1641

    Article  CAS  Google Scholar 

  6. Esposito E, Menegatti E, Cortesi R (2005) Hyaluronan-based microspheres as tools for drug delivery: a comparative study. Int J Pharm 288:35–49

    Article  CAS  Google Scholar 

  7. Huang WC, Chen SJ, Chen TL (2008) Production of hyaluronic acid by repeated batch fermentation. Biochem Eng J 40:460–464

    Article  CAS  Google Scholar 

  8. Hasegawa S, Nagatsuru M, Shibutani M, Yamamoto S, Hasebe S (1999) Productivity of concentrated hyaluronic acid using maxblend fermentor. J Biosci Bioeng 88:68–71

    Article  CAS  Google Scholar 

  9. Gao HJ, Du GC, Chen J (2006) Analysis of metabolic fluxes for hyaluronic acid (HA) production by Streptococcus zooepidemicus. World J Microbiol Biotechnol 22:399–408

    Article  CAS  Google Scholar 

  10. Liu L, Wang M, Du GC, Chen J (2008) Enhanced hyaluronic acid production of Streptococcus zooepidemicus by an intermittent alkaline-stress strategy. Lett Appl Microbiol 46:383–388

    Article  CAS  Google Scholar 

  11. Kim JH, Deok-Kun SY (1996) Selection of a Streptococcus equi mutant and optimization of culture conditions for the production of high molecular weight hyaluronic acid. Enzyme Microbiol Technol 19:440–445

    Article  CAS  Google Scholar 

  12. Kim SJ, Park SY, Kim CW (2006) A novel approach to the production of hyaluronic acid by Streptococcus zooepidemicus. J Microbiol Biotechnol 16:1849–1855

    CAS  Google Scholar 

  13. Liu L, Wang M, Du GC, Chen J, Sun J (2008) Influence of culture modes on the microbial production of hyaluronic acid by Streptococcus zooepidemicus. Biotechnol Bioproc E 13:269–273

    Article  CAS  Google Scholar 

  14. Liu L, Wang M, Du GC, Chen J, Sun J (2008) Enhanced hyaluronic acid production by a two-stage culture strategy based on the modeling of batch and fed-batch cultivation of Streptococcus zooepidemicus. Bioresour Technol 99:8532–8536

    Article  CAS  Google Scholar 

  15. Bitter H, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4:330–334

    Article  CAS  Google Scholar 

  16. Chisti Y, Jauregui-Haza UJ (2002) Oxygen transfer and mixing in mechanically agitated airlift bioreactors. Biochem Eng J 10:143–153

    Article  CAS  Google Scholar 

  17. Serrano CL, Corona RM, Sanchez A, Galindo E (1998) Prediction of xanthan fermentation development by a model linking kinetics, power drawn and mixing. Process Biochem 22:133–146

    Article  Google Scholar 

  18. Elibol M, Ozer D (2000) Influence of oxygen transfer on lipase production by Rhizopusarrhizus. Process Biochem 36:325–329

    Article  Google Scholar 

  19. Richard A, Margaritis A (2003) Rheology, oxygen transfer, and molecular weight characteristics of poly (glutamic acid) fermentation by Bacillus subtilis. Biotechnol Bioeng 89:299–305

    Article  Google Scholar 

  20. Rodríguez-Monroy M, Galindo E (1998) Broth rheology, growth and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shake flasks and in a stirred tank. Enzyme Microbiol Technol 24:687–693

    Article  Google Scholar 

  21. Casas JA, Santos VE, García-Ochoa F (2000) Xanthan gum production under several operational conditions: molecular structure and rheological properties. Enzyme Microbiol Technol 26:282–291

    Article  CAS  Google Scholar 

  22. Ryu DY, Humphrey AE (1972) A reassessment of oxygen transfer rates in antibiotics fermentations. J Ferm Technol 50:424–431

    Google Scholar 

  23. Yagi H, Yoshida F (1975) Gas absorption by Newtonian and non-Newtonian fluids in sparged agitated vessels. Ind Eng Chem Proc Des Dev 14:488–493

    Article  CAS  Google Scholar 

  24. Huang WC, Chen SJ, Chen TL (2006) The role of dissolved oxygen and function of agitation in hyaluronic acid fermentation. Biochem Eng J 32:239–243

    Article  CAS  Google Scholar 

  25. Liu L, Wang M, Du GC, Chen J, Sun J (2009) Microbial production of low molecular weight hyaluronic acid by adding hydrogen peroxide and ascorbate in batch culture of Streptococcus zooepidemicus. Bioresour Technol 100:362–367

    Article  CAS  Google Scholar 

  26. Cleary PP, Larkin A (1979) Hyaluronic acid capsule: strategy for oxygen resistance in group A Streptococci. J Bacteriol 140:1090–1097

    CAS  Google Scholar 

  27. Elibol M, Mavituna F (1999) A remedy to oxygen limitation problem in antibiotic production: addition of perfluorocarbon. Biochem Eng J 3:1–7

    Article  CAS  Google Scholar 

  28. Wilhelm E, Battino R (1986) The solubility of gases in liquids. 17. The solubility of gases in carbon tetrachloride. Chem Rev 73:214–220

    Google Scholar 

  29. Gotoh T, Mochizuki G, Kikuchi KI (2001) A novel column fermentor having a wetted-wall of perfluorocarbon as an oxygen carrier. Biochem Eng J 8:165–169

    Article  Google Scholar 

  30. Zhang JY, Ning Hao, Chen GQ (2006) Effect of expressing polyhydroxybutyrate synthesis genes (phbCAB) in streptococcus zooepidemicus on production of lactic acid and hyaluronic acid. Appl Microbiol Biotechnol 71:221–227

    Google Scholar 

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Acknowledgments

This project was financially supported by Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0532), the National Science Fund for Distinguished Young Scholars of China (No. 20625619), Program for Cultivation and Innovation of Graduate Students in Jiangsu Province (CX08B_128Z), and 973 Project (2007CB714306).

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Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, 214122, Wuxi, China

    Long Liu & Guocheng Du

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China

    Long Liu, Guocheng Du & Jian Chen

  3. State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China

    Jian Chen

  4. School of Food Science and Technology, Jiangnan University, 214122, Wuxi, China

    Miao Wang

  5. Institute of Information Technology, Jiangnan University, 214122, Wuxi, China

    Jun Sun

Authors
  1. Long Liu
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  2. Guocheng Du
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  3. Jian Chen
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  4. Miao Wang
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  5. Jun Sun
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Correspondence to Guocheng Du or Jian Chen.

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Liu, L., Du, G., Chen, J. et al. Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus . Bioprocess Biosyst Eng 32, 755–763 (2009). https://doi.org/10.1007/s00449-009-0300-6

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  • DOI: https://doi.org/10.1007/s00449-009-0300-6

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