Abstract
A higher Coenzyme Q10 (CoQ10) concentration of 25.04 mg/l was found in airlift bioreactor than the value of 18.11 mg/l obtained in stirred tank under the aerobic-dark cultivation of Rhodobacter sphaeroides. Aeration rate didn’t show obvious impact to CoQ10 production in airlift bioreactor. The fed-batch operation in airlift bioreactor could increase the biomass concentration and led to the maximum CoQ10 concentration of 33.91 mg/l measured, but a lower CoQ10 cell content (3.5 mg CoQ10/DCW) was observed in the fed-batch operation as compared to the batch operation. To enhance the CoQ10 content, an aeration-change strategy was proposed in the fed-batch operation of airlift bioreactor. This strategy led to the maximum CoQ10 concentration of 45.65 mg/l, a 35% increase as compared to the simple fed-batch operation. The results of this study suggested that a fed-batch operation in airlift bioreactor accompanying aeration-change could be suitable for CoQ10 production.
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References
Choi JH, Ryu YW, Seo JH (2005) Biotechnological production and applications of coenzyme Q10. Appl Microbiol Biotechnol 68:9–15
Kawamukai M (2002) Biosynthesis, bioproduction and novel roles of ubiquinone. J Biosci Bioeng 94:51–57
Lieberman A, Lyons K, Levine J, Myerburg R (2005) Statins, cholesterol, Co-enzyme Q10, and Parkinson’s disease. Parkinsonism Relat Disord 11:81–84
Cluis CP, Burja AM, Martin VJJ (2007) Current prospects for the production of coenzyme Q10 in microbes. TRENDS Biotechnol 25:514–521
Ha SJ, Kim SY, Seo JH, Oh DK, Lee JK (2007) Optimization of culture conditions and scale-up to pilot and plant scales for coenzyme Q10 production by Agrobacterium tumefaciens. Appl Microbiol Biotechnol 74:974–980
Yoshida H, Kotani Y, Ochiai K, Araki K (1998) Production of ubiquinone-10 using bacteria. J Gen Appl Microbiol 44:19–26
Kaplan P, Kucera I, Dadak V (1993) Effect of oxygen on ubiquinones-10 production by Paracoccus denitrificans. Biotechnol Lett 15:1001–1002
Sasaki K, Watanabe M, Suda Y, Ishizuka A, Noparatnaraporn N (2005) Applications of photosynthetic bacteria for medical fields. J Biosci Bioeng 100:481–488
Sakato K, Tanaka H, Shibata S, Kuratsu Y (1992) Agitation–aeartion studies on coenzyme Q10 production using Rhodopseudomonas spheroides. Biotechnol Appl Biochem 16:19–28
Yen HW, Chiu CH (2007) The influences of aerobic-dark and anaerobic-light cultivation on CoQ10 production by Rhodobacter sphaeroides in the submerged fermenter. Enzyme Microb Technol 41:600–604
Van’t Riet K (1979) Review of measuring methods and results in non-viscous gas–liquid mass transfer in stirred vessels. Ind Eng Chem Process Des Dev 18:357–364
Wee YJ, Kima JN, Yunb JS, Ryua HW (2004) Utilization of sugar molasses for economical l(+)-lactic acid production by batch fermentation of Enterococcus faecalis. Enzyme Microb Technol 34:568–573
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The authors wish to thank the National Science Council of the R.O.C. for financial supports (NSC 95-2221-E-029-007).
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Yen, HW., Shih, TY. Coenzyme Q10 production by Rhodobacter sphaeroides in stirred tank and in airlift bioreactor. Bioprocess Biosyst Eng 32, 711–716 (2009). https://doi.org/10.1007/s00449-008-0294-5
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DOI: https://doi.org/10.1007/s00449-008-0294-5