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Pectinase partitioning in polyethylene glycol 1000/Na2SO4 aqueous two-phase system: statistical modeling of the experimental results

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Abstract

Statistical models concerning partitioning of pectinase in polyethylene glycol 1000/Na2SO4 aqueous two-phase system were established with response surface methodology. Concentrations of polyethylene glycol 1000 and Na2SO4 were selected as independent variables to evaluate their impact on parameters of partitioning in aqueous two-phase system—the partition coefficient of pectinase, purification factor and pectinase yield. An experimental space where over 2.5-fold purification was achieved, followed by over 90% yield of pectinase. The established models showed good prediction of partitioning parameters.

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Acknowledgments

This work was supported by grant number 142 046 from Ministry of Science, Republic of Serbia. Authors wish to thank Ms. Senka Mađarev for her excellent technical assistance.

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  1. Faculty of Technology, University of Novi Sad, Blvd. Cara Lazara 1, Novi Sad, Serbia

    Mirjana Antov & Radovan Omorjan

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  1. Mirjana Antov
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  2. Radovan Omorjan
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Correspondence to Mirjana Antov.

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Antov, M., Omorjan, R. Pectinase partitioning in polyethylene glycol 1000/Na2SO4 aqueous two-phase system: statistical modeling of the experimental results. Bioprocess Biosyst Eng 32, 235–240 (2009). https://doi.org/10.1007/s00449-008-0243-3

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  • DOI: https://doi.org/10.1007/s00449-008-0243-3

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