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An amine: hydroxyacetone aminotransferase from Moraxella lacunata WZ34 for alaninol synthesis

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Abstract

An amine:hydroxyacetone aminotransferase from an isolated soil bacterium, Moraxella lacunata WZ34, was employed to synthesize alaninol in the presence of hydroxyacetone and isopropylamine in this study. The optimal carbon and nitrogen sources were glycerol and beef extract, respectively. A wide range of amino donor specificity was detected with the aminotransferase, which exhibited a relative high activity (9.83 U mL−1) in the presence of isopropylamine. The enzyme was the most active at pH 8.5, and showed relatively higher activity at alkaline than acidic pH. Maximum activity was achieved at 30 °C, and the enzyme had good thermal stability below 60 °C. Metal ions such as Mg2+ had positive effect (132.6%) on the enzyme, and (aminooxy)acetic acid, a typical aminotransferase inhibitor, significantly inhibited its activity. The enzyme activity was enhanced by the addition of 0.05 mM pyridoxal-5′-phosphate (PLP).

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Authors and Affiliations

  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China

    Dongzhi Chen, Zhao Wang, Yinjun Zhang, Zeyu Sun & Qin Zhu

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  1. Dongzhi Chen
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  2. Zhao Wang
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  3. Yinjun Zhang
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  4. Zeyu Sun
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  5. Qin Zhu
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Correspondence to Zhao Wang.

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Chen, D., Wang, Z., Zhang, Y. et al. An amine: hydroxyacetone aminotransferase from Moraxella lacunata WZ34 for alaninol synthesis. Bioprocess Biosyst Eng 31, 283–289 (2008). https://doi.org/10.1007/s00449-007-0158-4

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  • DOI: https://doi.org/10.1007/s00449-007-0158-4

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