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Comparative profiles of α-amylase production in conventional tray reactor and GROWTEK bioreactor

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Abstract

GROWTEK bioreactor was used as modified solid-state fermentor to circumvent many of the problems associated with the conventional tray reactors for solid-state fermentation (SSF). Aspergillus oryzae IFO-30103 produced very high levels of α-amylase by modified solid-state fermentation (mSSF) compared to SSF carried out in enamel coated metallic trays utilizing wheat bran as substrate. High α-amylase yield of 15,833 U g−1 dry solid in mSSF were obtained when the fungus were cultivated at an initial pH of 6.0 at 32°C for 54 h whereas α-amylase production in SSF reached its maxima (12,899 U g−1 dry solid ) at 30°C after 66 h of incubation. With the supplementation of 1% NaNO3, the maximum activity obtained was 19,665 U g−1 dry solid (24% higher than control) in mSSF, whereas, in SSF maximum activity was 15,480 U g−1 dry solid in presence of 0.1% Triton X-100 (20% higher than the control).

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Acknowledgment

Financial support from the Department of Biotechnology, New Delhi, India, is gratefully acknowledged.

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Authors and Affiliations

  1. Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India

    Tapati Bhanja, S. Rout & Rintu Banerjee

  2. Department of Food Technology, Techno India Group, Salt Lake City, Sector 5, Kolkata, India

    B. C. Bhattacharyya

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  1. Tapati Bhanja
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  2. S. Rout
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  3. Rintu Banerjee
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  4. B. C. Bhattacharyya
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Correspondence to Rintu Banerjee.

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Bhanja, T., Rout, S., Banerjee, R. et al. Comparative profiles of α-amylase production in conventional tray reactor and GROWTEK bioreactor. Bioprocess Biosyst Eng 30, 369–376 (2007). https://doi.org/10.1007/s00449-007-0133-0

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  • DOI: https://doi.org/10.1007/s00449-007-0133-0

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