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Evaluation of a novel Wave Bioreactor® cellbag for aerobic yeast cultivation

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Abstract

The Wave Bioreactor® is widely used in cell culture due to the benefits of disposable technology and ease of use. A novel cellbag was developed featuring a frit sparger to increase the system's oxygen transfer. The purpose of this work was to evaluate the sparged cellbag for yeast cultivation. Oxygen mass transfer studies were conducted in simulated culture medium and the sparged system's maximum oxygen mass transfer coefficient (k L a) was 38 h-1. These measurements revealed that the sparger was ineffective in increasing the oxygen transfer capacity. Cultures of Saccharomyces cerevisiae were successfully grown in oxygen-blended sparged and oxygen-blended standard cellbags. Under steady state conditions for both cellbag designs, k L a values as high as 60 h-1 were obtained with no difference in growth characteristics. This is the first report of a successful cultivation of a microbe in a Wave Bioreactor® comparing conventional seed expansion in shake flasks and stirred tank bioreactors.

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Abbreviations

C*:

saturated dissolved oxygen concentration in the liquid phase (mmol L−1)

C 1 :

dissolved oxygen concentration at time t 1 (mmol L−1)

C 2 :

dissolved oxygen concentration at time t 2 (mmol L−1)

CER:

carbon dioxide evolution rate (mmol L−1 h−1)

DO:

dissolved oxygen (% air sat)

k L a :

volumetric oxygen transfer coefficient (h−1)

OD:

optical density

OTR:

oxygen transfer rate (mmol L−1 h−1)

OUR:

oxygen uptake rate (mmol L−1 h−1)

PBS:

phosphate buffered saline

RPM:

rocks per minute (min−1)

RQ:

respiration quotient (CER/OUR)

s :

substrate (g L−1)

vvm:

volume of gas sparge per volume liquid per minute (L L−1 min−1)

Y x/s :

yield coefficient, g biomass/g glucose

x :

biomass (g)

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Acknowledgments

The authors would like to acknowledge and thank Dr. Arthur L. Kruckeberg for supplying the AlcoFree® strain and for useful discussions.

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Authors and Affiliations

  1. Fermentation and Cell Culture, Merck and Co., P.O. Box 4, Mailstop WP26C-1, West Point, PA, 19486, USA

    Mark Mikola, Jennifer Seto & Ashraf Amanullah

Authors
  1. Mark Mikola
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  2. Jennifer Seto
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  3. Ashraf Amanullah
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Correspondence to Mark Mikola.

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Mikola, M., Seto, J. & Amanullah, A. Evaluation of a novel Wave Bioreactor® cellbag for aerobic yeast cultivation. Bioprocess Biosyst Eng 30, 231–241 (2007). https://doi.org/10.1007/s00449-007-0119-y

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  • DOI: https://doi.org/10.1007/s00449-007-0119-y

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