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Polyhydric alcohol protective effect on Rhizomucor miehei lipase deactivation enhanced by pressure and temperature treatment

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Abstract

The influence of polyhydric alcohols (sorbitol, xylitol, erythritol, glycerol) on the thermal stability of Rhizomucor miehei lipase has been studied at high hydrostatic pressure (up to 500 MPa). In the absence of additives, a protective effect (PE) (the ratio between the residual activities determined at 480 MPa for the enzyme in the presence or absence of polyhydric alcohols) of low-applied pressures (from 50 MPa to 350 MPa) against thermal deactivations (at 50°C and 55°C) has been noticed. In the presence of additives, a strong correlation between PE and the total hydroxyl group concentration has been obtained, for the first time, under treatments of combining denaturing temperatures and high hydrostatic pressures. This relationship does not seem to be dependent on the nature polyhydric alcohols as the same effect could be observed with 1 M sorbitol and 2 M glycerol. This PE, against thermal and high pressure combined lipase deactivation, increases with polyhydric alcohol concentrations, and when temperature increases from 25°C to 55°C.

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Abbreviations

C n :

n carbon atoms

kDa:

Molecular weight unit (103 Da)

M:

Molar concentration unit (mol.l−1)

MPa:

Pressure unit (106 P=10 bars)

OH group:

Hydroxyl group

PE:

Protective effect

PME:

Pectin methylesterase

P50% :

Pressure of half deactivation for a settled treatment time (MPa)

RML:

Rhizomucor miehei lipase

t 1/2 :

Half-life (min)

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Acknowledgments

Marilyne Noël (pHD student) and authors are very grateful to Peter Winterton for improving the English of the manuscript.

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Authors and Affiliations

  1. INSA Centre de Bioingénierie Gilbert Durand, 135 avenue de Rangueil, 31077, Toulouse cedex 4, France

    Marilyne Noël & Didier Combes

  2. Departamento de Bioquímica y Biología Molecular B e Inmunología. Facultad de Química, Universidad de Murcia, P.O. Box 4021, Campus de Espinardo, Murcia, Spain

    Pedro Lozano

Authors
  1. Marilyne Noël
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  2. Pedro Lozano
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  3. Didier Combes
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Correspondence to Didier Combes.

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Noël, M., Lozano, P. & Combes, D. Polyhydric alcohol protective effect on Rhizomucor miehei lipase deactivation enhanced by pressure and temperature treatment. Bioprocess Biosyst Eng 27, 375–380 (2005). https://doi.org/10.1007/s00449-005-0417-1

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  • DOI: https://doi.org/10.1007/s00449-005-0417-1

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