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An isotopic method for testing the influence of leaf litter quality on carbon fluxes during decomposition

  • Ecosystem Ecology
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Abstract

During microbial breakdown of leaf litter a fraction of the C lost by the litter is not released to the atmosphere as CO2 but remains in the soil as microbial byproducts. The amount of this fraction and the factors influencing its size are not yet clearly known. We performed a laboratory experiment to quantify the flow of C from decaying litter into the soil, by means of stable C isotopes, and tested its dependence on litter chemical properties. Three sets of 13C-depleted leaf litter (Liquidambar styraciflua L., Cercis canadensis L. and Pinus taeda L.) were incubated in the laboratory in jars containing 13C-enriched soil (i.e. formed C4 vegetation). Four jars containing soil only were used as a control. Litter chemical properties were measured using thermogravimetry (Tg) and pyrolysis–gas chromatography/mass spectrometry–combustion interface–isotope ratio mass spectrometry (Py–GC/MS–C–IRMS). The respiration rates and the δ13C of the respired CO2 were measured at regular intervals. After 8 months of incubation, soils incubated with both L. styraciflua and C. canadensis showed a significant change in δ13C (δ13Cfinal = −20.2 ± 0.4‰ and −19.5 ± 0.5‰, respectively) with respect to the initial value (δ13Cinitial = −17.7 ± 0.3‰); the same did not hold for soil incubated with P. taeda (δ13Cfinal:−18.1 ± 0.5‰). The percentages of litter-derived C in soil over the total C loss were not statistically different from one litter species to another. This suggests that there is no dependence of the percentage of C input into the soil (over the total C loss) on litter quality and that the fractional loss of leaf litter C is dependent only on the microbial assimilation efficiency. The percentage of litter-derived C in soil was estimated to be 13 ± 3% of total C loss.

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Acknowledgements

We would like to thank ESF (SIBAE program) for a grant given to Mauro Rubino to collaborate at the Max Planck Institute for Biogeochemistry (Jena). The work was partially supported by the EU’s CARBOEUROPE IP. We would also like to thank Federica Farina for her help in the lab and the anonymous reviewers whose suggestions greatly improved the original manuscript. To the best of their knowledge, the authors of the present manuscript declare that the experiment described above complied with Italian law.

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Authors and Affiliations

  1. Dipartimento di Scienze Ambientali, Seconda Università di Napoli, Via Vivaldi 43, 81100, Caserta, Italy

    Mauro Rubino, C. Lubritto, A. D’Onofrio, F. Terrasi & M. F. Cotrufo

  2. Max Planck Institute for Biogeochemistry, Winzerlaer Strasse 10, 07745, Jena, Germany

    G. Gleixner

  3. Unite UR1119 Unite Geochimie des Sols et des Eaux, INRA, 13545, Aix en Provence, France

    Mauro Rubino

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  1. Mauro Rubino
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  2. C. Lubritto
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  3. A. D’Onofrio
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  4. F. Terrasi
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  5. G. Gleixner
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  6. M. F. Cotrufo
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Correspondence to Mauro Rubino.

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Communicated by Dan Yakir.

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Rubino, M., Lubritto, C., D’Onofrio, A. et al. An isotopic method for testing the influence of leaf litter quality on carbon fluxes during decomposition. Oecologia 154, 155–166 (2007). https://doi.org/10.1007/s00442-007-0815-y

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