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Connecting plant–microbial interactions above and belowground: a fungal endophyte affects decomposition

  • Ecosystem Ecology
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Abstract

Mutualisms can strongly affect the structure of communities, but their influence on ecosystem processes is not well resolved. Here we show that a plant–microbial mutualism affects the rate of leaf litter decomposition using the widespread interaction between tall fescue grass (Lolium arundinaceum) and the fungal endophyte Neotyphodium coenophialum. In grasses, fungal endophytes live symbiotically in the aboveground tissues, where the fungi gain protection and nutrients from their host and often protect host plants from biotic and abiotic stress. In a field experiment, decomposition rate depended on a complex interaction between the litter source (collected from endophyte-infected or endophyte-free plots), the decomposition microenvironment (endophyte-infected or endophyte-free plots), and the presence of mesoinvertebrates (manipulated by the mesh size of litter bags). Over all treatments, decomposition was slower for endophyte-infected fescue litter than for endophyte-free litter. When mesoinvertebrates were excluded using fine mesh and litter was placed in a microenvironment with the endophyte, the difference between endophyte-infected and endophyte-free litter was strongest. In the presence of mesoinvertebrates, endophyte-infected litter decomposed faster in microenvironments with the endophyte than in microenvironments lacking the endophyte, suggesting that plots differ in the detritivore assemblage. Indeed, the presence of the endophyte in plots shifted the composition of Collembola, with more Hypogastruridae in the presence of the endophyte and more Isotomidae in endophyte-free plots. In a separate outdoor pot experiment, we did not find strong effects of the litter source or the soil microbial/microinvertebrate community on decomposition, which may reflect differences between pot and field conditions or other differences in methodology. Our work is among the first to demonstrate an effect of plant–endophyte mutualisms on ecosystem processes under field conditions.

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Acknowledgements

Many thanks to Joseph Reznik for assistance identifying Collembola, Jessica Stuaan for sorting Collembola, Jon Dahl for tissue analysis, Keenan Mack and Brette Thompson for help in the field, and two anonymous reviewers for helpful comments on the manuscript. This research was funded by a Howard Hughes Medical Institute Undergraduate Research Capstone Award to A.L., by NSF DBI-0200485 to J.A.R., and by NSF DEB-9727116 to K.C. All experiments complied with the current laws of the USA.

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  1. Department of Biology, Indiana University, Bloomington, IN, 47408, USA

    Alisha Lemons, Keith Clay & Jennifer A. Rudgers

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  1. Alisha Lemons
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  3. Jennifer A. Rudgers
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Correspondence to Alisha Lemons.

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Communicated by Alan Knapp

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Lemons, A., Clay, K. & Rudgers, J. Connecting plant–microbial interactions above and belowground: a fungal endophyte affects decomposition. Oecologia 145, 595–604 (2005). https://doi.org/10.1007/s00442-005-0163-8

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