Differential effects of a labial mutation on the development, structure, and function of stomach acid-secreting cells in Drosophila melanogaster larvae and adults

Abstract.

The differentiation of copper cells, which secrete stomach acid in Drosophila larvae, has been shown previously to be sensitive to the labial ^k3 mutation. Here we found that stomach acid secretion in adults was insensitive to lab ^k3. The basis for this stage-specific effect was elucidated by characterizing the development, structure, and function of the adult midgut. First, we demonstrated by copper-dependent fluorescence and morphology that copper cells were present in the adult stomach. Fine-structure analysis of adult copper cells led to the identification of a previously unrecognized plasma membrane domain: apicolateral contacts between copper cells and their neighbors consisted of smooth septate junctions that were enriched in αβ-spectrin and ankyrin. Second, we demonstrated that adult copper cells were present in lab ^k3/lab ^vd1 (conditional/null) adults. The labial protein was expressed in adult lab ^k3/lab ^vd1 copper cells, but not in larvae. Thus the lab ^k3 mutation had a stage-specific effect on midgut labial expression, but did not appear to affect protein function. Surprisingly, stomach acidification was dispensable during larval development, since lab ^k3/lab ^vd1 mutant larvae that lacked midgut acidification developed into fertile adults.