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Cd271 mediates proliferation and differentiation of epidermal stem cells to support cutaneous burn wound healing

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Abstract

Burn wounds can significantly reduce the quality of life of patients with respect to their physiology and psychology and can even threaten their lives. Many treatments have been proposed, including stem cell therapy but no effective method can as yet cure such damage. Our study highlights the role of Cd271 in epidermal stem cells (eSC) during the healing of burn wounds. The expression of Cd271 increases together with burn wound healing. Injection of Cd271-over-expressing eSC into wounds promotes the healing rate in a mouse burn model. Over-expression of Cd271 enhances the abilities of eSC with regard to their differentiation, proliferation and migration and even their resistance to apoptosis in vitro. These results are in accordance with a hypothesis suggesting that Cd271 promotes the healing of skin burn wounds by improving the potential of eSC for differentiation, proliferation and migration. Our findings shed light on the role of Cd271 in wound healing and may provide new therapeutic approaches for curing burn wounds of the skin.

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Abbreviations

Krt:

Cytokeratin

EGFR:

Epidermal growth factor receptor

eSC:

Epidermal stem cells

GAPDH:

Glyceraldehydes-3-phosphate dehydrogenase

KC:

Keratinocyte

Ngf:

Nerve growth factor

NT:

Neurotrophin

FBS:

Fetal bovine serum

PI:

Propidium iodide

TAC:

Transit amplifying cells

CCK-8:

Cell Counting Kit-8

IHC:

Immunohistochemistry

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Authors and Affiliations

  1. Department of Burns and Plastic Surgery, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, 250000, China

    Min Zhang, Yongqian Cao, Ran Zhao, Mingqing Wang, Wenjun Xue, Zhanjie Yang & Yibing Wang

  2. Health Management Center, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, 250000, China

    Xiaohong Li

  3. School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China

    Lizhi Hu, Sahbi Khaled Taieb, Xiaolong Zhu & Jing Zhang

  4. Department of Laser Aesthetic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing, 100000, China

    Yongqiang Feng

Authors
  1. Min Zhang
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  2. Yongqian Cao
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  3. Xiaohong Li
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  4. Lizhi Hu
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  5. Sahbi Khaled Taieb
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  6. Xiaolong Zhu
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  7. Jing Zhang
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  8. Yongqiang Feng
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  9. Ran Zhao
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  11. Wenjun Xue
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  12. Zhanjie Yang
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  13. Yibing Wang
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Contributions

Yibing Wang, Min Zhang and Yongqian Cao conceived and designed the experiments; Min Zhang performed the experiments; Yongqian Cao and Xiaohong Li contributed reagents, materials and analysis tools; Yongqiang Feng, Ran Zhao, Mingqing Wang, Wenjun Xue and Zhanjie Yang analysed the data; Min Zhang, Xiaohong Li and Lizhi Hu wrote the paper; Xiaohong Li and Jun Ding reviewed the paper; Sahbi Khaled Taieb, Jing Zhang and Xiaolong Zhu reviewed the references. This study was funded by the National Natural Science Foundation of China (nos. 81571911, 81201471 and 81171818) and the Science and Technology Development Program of Shandong Province (no. 2015GSF118008).

Corresponding author

Correspondence to Yibing Wang.

Ethics declarations

This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Shandong University Laboratory Animal Centre. The protocol was approved by the Committee on the Ethics of Shandong University. All surgery was performed under pentobarbital sodium anaesthesia and all efforts were made to minimize suffering.

Conflict of interests

The authors declare no conflict of interest.

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Zhang, M., Cao, Y., Li, X. et al. Cd271 mediates proliferation and differentiation of epidermal stem cells to support cutaneous burn wound healing. Cell Tissue Res 371, 273–282 (2018). https://doi.org/10.1007/s00441-017-2723-8

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  • DOI: https://doi.org/10.1007/s00441-017-2723-8

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