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Recovery of renal function after administration of adipose-tissue-derived stromal vascular fraction in rat model of acute kidney injury induced by ischemia/reperfusion injury

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Abstract

Acute kidney injury (AKI) induced by ischemia/reperfusion (I/R) injury is a major challenge in critical care medicine. The purpose of this study is to determine the therapeutic effects of the adipose-tissue-derived stromal vascular fraction (SVF) and the optimal route for SVF delivery in a rat model of AKI induced by I/R injury. Fifty male Sprague–Dawley rats were randomly divided into five groups (10 animals per group): sham, nephrectomy control, I/R injury control, renal arterial SVF infusion and subcapsular SVF injection. To induce AKI by I/R injury, the left renal artery was clamped with a nontraumatic vascular clamp for 40 min, and the right kidney was removed. Rats receiving renal arterial infusion of SVF had a significantly reduced increase in serum creatinine compared with the I/R injury control group at 4 days after I/R injury. The glomerular filtration rate of the renal arterial SVF infusion group was maintained at a level similar to that of the sham and nephrectomy control groups at 14 days after I/R injury. Masson’s trichrome staining showed significantly less fibrosis in the renal arterial SVF infusion group compared with that in the I/R injury control group in the outer stripe (P < 0.001). TUNEL labeling showed significantly decreased apoptosis in both the renal arterial SVF infusion and subcapsular SVF injection groups compared with the I/R injury control group in the outer stripe (P < 0.001). Thus, renal function is effectively rescued from AKI induced by I/R injury through the renal arterial administration of SVF in a rat model.

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Abbreviations

AKI:

Acute kidney injury

DMEM:

Dulbecco’s modified Eagle’s medium

FBS:

Fetal bovine serum

GFR:

Glomerular filtration rate

GPx:

Glutathione peroxidase

GR:

Glutathione reductase

I/R:

Ischemia/reperfusion

PBS:

Phosphate-buffered saline

SVF:

Stromal vascular fraction

TUNEL:

Terminal-deoxynucleotidyl-transferase-mediated dUTP nick-end labeling

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Authors and Affiliations

  1. Department of Urology, Gyeongsang National University Changwon Hospital, Gyeongsang National University School of Medicine, Changwon, South Korea

    Chunwoo Lee

  2. Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea

    Myoung Jin Jang

  3. Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-Gil, Songpa-gu, Seoul, 05505, South Korea

    Bo Hyun Kim, Jin Young Park, Dalsan You, In Gab Jeong, Jun Hyuk Hong & Choung-Soo Kim

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  1. Chunwoo Lee
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  2. Myoung Jin Jang
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  3. Bo Hyun Kim
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  4. Jin Young Park
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Correspondence to Choung-Soo Kim.

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Lee, C., Jang, M.J., Kim, B.H. et al. Recovery of renal function after administration of adipose-tissue-derived stromal vascular fraction in rat model of acute kidney injury induced by ischemia/reperfusion injury. Cell Tissue Res 368, 603–613 (2017). https://doi.org/10.1007/s00441-017-2585-0

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