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Complex morphology of gastrin-releasing G-cells in the antral region of the mouse stomach

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Abstract

Gastrin-releasing enteroendocrine cells (G-cells) are usually described as flask-shaped cells with a large base and a small apical pole, integrated in the epithelium lining the basal region of the antral invaginations in the stomach. By means of a transgenic mouse line in which the enhanced version of GFP is endogenously expressed under the control of a gastrin promoter, we have analyzed the spatial distribution and morphological features of G-cells. We found that G-cells were not only located at the basal region of the invagination but to a lesser extent also at the upper region. Visualization of the entire cellular morphology revealed that G-cells show complex morphologies. Basally located G-cells are roundish-shaped cells which project a prominent apical process towards the lumen and extend basal protrusions containing the hormone gastrin that were frequently found in close proximity to blood vessels and occasionally in the vicinity of nerve fibers. Inspection of G-cells in the upper region of antral invaginations disclosed a novel population of G-cells. These cells have a spindle-like contour and long apical and basal processes which extend vertically along the antral invagination, parallel to the lumen. This G-cell population seems to be in contact with a network of nerve fibers. While the functional role of these untypical G-cells is still elusive, the results of this study provide some useful indications to possible roles of these G-cells.

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Acknowledgments

The authors thank Kerstin Bach for excellent technical assistance and Dr. Timothy C. Wang for providing the mGas-EGFP mice. This work was supported by the Deutsche Forschungsgemeinschaft, BR 712/25-1.

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    Authors and Affiliations

    1. Institute of Physiology, University of Hohenheim, Garbenstr. 30, 70599, Stuttgart, Germany

      Claudia Frick, Amelie Therese Rettenberger, Malena Luisa Lunz & Heinz Breer

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    1. Claudia Frick
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    2. Amelie Therese Rettenberger
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    3. Malena Luisa Lunz
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    4. Heinz Breer
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    Correspondence to Claudia Frick.

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    Claudia Frick and Amelie Therese Rettenberger contributed equally to this work.

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    Frick, C., Rettenberger, A.T., Lunz, M.L. et al. Complex morphology of gastrin-releasing G-cells in the antral region of the mouse stomach. Cell Tissue Res 366, 301–310 (2016). https://doi.org/10.1007/s00441-016-2455-1

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    • DOI: https://doi.org/10.1007/s00441-016-2455-1

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