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MyoD promotes porcine PPARγ gene expression through an E-box and a MyoD-binding site in the PPARγ promoter region

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Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) is a key transcription factor in adipogenesis and can be regulated by adipogenesis-related factors. However, little information is available regarding its regulation by myogenic factors. In this study, we found that over-expression of MyoD enhanced porcine adipocyte differentiation and up-regulated PPARγ expression, whereas small interfering RNA against MyoD significantly attenuated porcine adipocyte differentiation and inhibited PPARγ expression. The MyoD-binding sites in the PPARγ promoter region at −412 to −396 and −155 to −150 were identified by promoter deletion analysis and site-directed mutagenesis. Electrophoretic mobility shift assays and chromatin immunoprecipitation further showed that these two regions are MyoD-binding sites, both in vitro and in vivo, indicating that MyoD directly interacts with the porcine PPARγ promoter. Thus, our results demonstrate that an Enhancer box and a binding site for a cooperative co-activator of MyoD are present in the promoter region of porcine PPARγ; furthermore, MyoD up-regulates PPARγ expression and promotes porcine adipocyte differentiation.

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    Authors and Affiliations

    1. Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei, 430208, Peoples Republic of China

      Bing Deng, Haijun Huang, Wu Liu, Shengqiang Ye, Lixia Wang, Yu Yang & Ping Gong

    2. Key Laboratory of Swine Genetics and Breeding of the Agricultural Ministry and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Peoples Republic of China

      Feng Zhang, Kun Chen & Siwen Jiang

    3. Wuhan University of Technology, Wuhan, 430074, Peoples Republic of China

      Jianghui Wen

    4. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Peoples Republic of China

      Siwen Jiang

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    1. Bing Deng
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    2. Feng Zhang
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    Correspondence to Siwen Jiang.

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    Bing Deng and Feng Zhang contributed equally to this work.

    This research was supported by the National Natural Science Foundation of China (31201766, 31472075 and 31272425), the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (2014BAD20B01 and 2011BAD28B01) and the Science and Technology Planning Project of Hubei Province, China (2014ABA025).

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    Deng, B., Zhang, F., Chen, K. et al. MyoD promotes porcine PPARγ gene expression through an E-box and a MyoD-binding site in the PPARγ promoter region. Cell Tissue Res 365, 381–391 (2016). https://doi.org/10.1007/s00441-016-2380-3

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    • DOI: https://doi.org/10.1007/s00441-016-2380-3

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