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Advances in chemical labeling of proteins in living cells

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Abstract

The pursuit of quantitative biological information via imaging requires robust labeling approaches that can be used in multiple applications and with a variety of detectable colors and properties. In addition to conventional fluorescent proteins, chemists and biologists have come together to provide a range of approaches that combine dye chemistry with the convenience of genetic targeting. This hybrid-tagging approach amalgamates the rational design of properties available through synthetic dye chemistry with the robust biological targeting available with genetic encoding. In this review, we discuss the current range of approaches that have been exploited for dye targeting or for targeting and activation and some of the recent applications that are uniquely permitted by these hybrid-tagging approaches.

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Authors and Affiliations

  1. Sharp Edge Laboratories, Pittsburgh, Pa., USA

    Qi Yan & Marcel P. Bruchez

  2. Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pa., USA

    Marcel P. Bruchez

  3. Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pa., USA

    Marcel P. Bruchez

  4. Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pa., USA

    Marcel P. Bruchez

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  1. Qi Yan
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Correspondence to Marcel P. Bruchez.

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Yan, Q., Bruchez, M.P. Advances in chemical labeling of proteins in living cells. Cell Tissue Res 360, 179–194 (2015). https://doi.org/10.1007/s00441-015-2145-4

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