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Resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells

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Abstract

A large population of perivascular cells was found to be present in the area of the blood-labyrinth barrier in the stria vascularis of normal adult cochlea. The cells were identified as perivascular resident macrophages (PVMs), as they were positive for several macrophage surface molecules including F4/80, CD68, and CD11b. The macrophages, which were closely associated with microvessels and structurally intertwined with endothelial cells and pericytes, constitutively expressed scavenger receptor classes A1 and B1 and accumulated blood-borne proteins such as horseradish peroxidase and acetylated low-density lipoprotein. The PVMs were demonstrated to proliferate slowly, as evidenced by the absence of 5-bromo-2-deoxyuridine (BrdU)-positive PVMs at 3–14 days in normal mice injected with BrdU. However, in irradiated mice, the majority of the PVMs turned over via bone-marrow-cell migration within a 10-month time-frame. The existence of PVMs in the vascular wall of the blood-labyrinth barrier might therefore serve as a source for progenitor cells for postnatal vasculogenesis and might contribute to the repair of damaged vessels in the context of a local inflammatory response.

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Acknowledgements

I thank Drs. Dai Min and Yang Yue (Oregon Hearing Research Center, Oregon Health & Science University) for their assistance with bone-marrow-cell transplantation and injection of BrdU. I am also grateful to Dr. Alfred Nuttall (Oregon Hearing Research Center, Oregon Health & Science University) for his critical review and valuable discussion of the manuscript. Finally, my thanks are extended to Janice Moore and Elizabeth Servin for their editorial help with this manuscript.

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  1. Oregon Hearing Research Center, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, NRC04, Portland, OR 97239, USA

    Xiaorui Shi

  2. The Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

    Xiaorui Shi

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  1. Xiaorui Shi
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Correspondence to Xiaorui Shi.

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This work was supported by the National Institute of Deafness and Other Communications Disorders (grants R01 DC00844, R03 DC008888-02, and P30 DC005983).

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Shi, X. Resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells. Cell Tissue Res 342, 21–30 (2010). https://doi.org/10.1007/s00441-010-1040-2

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