Skip to main content

Advertisement

SpringerLink
Log in

Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels

  • Regular Article
  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Abstract

Advances in bioengineering, material chemistry, and developmental biology have led to the design of three-dimensional (3D) culture systems that better resemble the surrounding structure and chemistry of the in situ niches of cells in tissues. This study was designed to characterize and compare porcine adipose-derived stem cells (ADSC) and bone-marrow-derived stem cells (BMSC) induced to differentiate toward osteogenic and adipogenic lineages in vitro by using a 3D alginate hydrogel. The morphology and gene expression of the two cell populations during differentiation were analyzed. Both ADSC and BMSC showed morphological evidence of osteogenic and adipogenic differentiation. Expression patterns of genes characteristic of the onset of osteogenic differentiation (ALP, COL1A1, SPARC, SPP1) were low at the beginning of culture and generally increased during the period of differentiation up to 28 days in culture. Expression of genes associated with adipogenic differentiation (ACSL1, ADFP, ADIPOQ, CD36, DBI, DGAT2, PPARG, SCD) was consistently increased in ADSC cultured in alginate hydrogel relative to the start of differentiation. However, adipogenic gene expression of BMSC cultured in alginate hydrogel was more limited when compared with that of ADSC. Evaluation of cell numbers (via the MTT staining assay) suggested a greater viability of BMSC under osteogenic conditions in alginate hydrogels than under adipogenic conditions, whereas ADSC had greater viability under adipogenic conditions than under osteogenic conditions. This study thus provides an important initial evaluation of ADSC and BMSC seeded and differentiated toward the osteogenic and adipogenic cell lineages in a 3D alginate hydrogel in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Alam HB, Duggan M, Li Y, Spaniolas K, Liu B, Tabbara M, Demoya M, Sailhamer EA, Shults C, Velmahos GC (2008) Putting life on hold—for how long? Profound hypothermic cardiopulmonary bypass in a swine model of complex vascular injuries. J Trauma 64:912–922

    Article  PubMed  Google Scholar 

  • Andreeff M, Goodrich DW, Pardee AB (2003) Cancer medicine 6. Decker, Hamilton

    Google Scholar 

  • Augst AD, Kong HJ, Mooney DJ (2006) Alginate hydrogels as biomaterials. Macromol Biosci 6:623–633

    Article  CAS  PubMed  Google Scholar 

  • Biname F, Pawlak G, Roux P, Hibner U (2010) What makes cells move: requirements and obstacles for spontaneous cell motility. Mol Biosyst 6:648–661

    Article  CAS  PubMed  Google Scholar 

  • Burdick JA, Vunjak-Novakovic G (2009) Engineered microenvironments for controlled stem cell differentiation. Tissue Eng A 15:205–219

    Article  CAS  Google Scholar 

  • Cinquin O (2009) Purpose and regulation of stem cells: a systems-biology view from the Caenorhabditis elegans germ line. J Pathol 217:186–198

    Article  PubMed  Google Scholar 

  • Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317

    Article  CAS  PubMed  Google Scholar 

  • Duggal S, Fronsdal KB, Szoke K, Shahdadfar A, Melvik JE, Brinchmann JE (2009) Phenotype and gene expression of human mesenchymal stem cells in alginate scaffolds. Tissue Eng A 15:1763–1773

    Article  CAS  Google Scholar 

  • Even-Ram S, Yamada KM (2005) Cell migration in 3D matrix. Curr Opin Cell Biol 17:524–532

    Article  CAS  PubMed  Google Scholar 

  • Fraser JK, Wulur I, Alfonso Z, Hedrick MH (2006) Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 24:150–154

    Article  CAS  PubMed  Google Scholar 

  • Ghidoni I, Chlapanidas T, Bucco M, Crovato F, Marazzi M, Vigo D, Torre ML, Faustini M (2008) Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine. Cytotechnology 58:49–56

    Article  CAS  PubMed  Google Scholar 

  • Halberstadt C, Austin C, Rowley J, Culberson C, Loebsack A, Wyatt S, Coleman S, Blacksten L, Burg K, Mooney D, Holder W Jr (2002) A hydrogel material for plastic and reconstructive applications injected into the subcutaneous space of a sheep. Tissue Eng 8:309–319

    Article  CAS  PubMed  Google Scholar 

  • Hoehn M, Kustermann E, Blunk J, Wiedermann D, Trapp T, Wecker S, Focking M, Arnold H, Hescheler J, Fleischmann BK, Schwindt W, Buhrle C (2002) Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proc Natl Acad Sci USA 99:16267–16272

    Article  CAS  PubMed  Google Scholar 

  • Jing W, Lin Y, Wu L, Li X, Nie X, Liu L, Tang W, Zheng X, Tian W (2007) Ectopic adipogenesis of preconditioned adipose-derived stromal cells in an alginate system. Cell Tissue Res 330:567–572

    Article  PubMed  Google Scholar 

  • Kong HJ, Lee KJ, Mooney DJ (2002) Decoupling effects of solids concentration on rheologycal properties of pre-geled alginate solution and mechanical propertities of post hydrogel by utilizing binary molecular weight distribution. Polymer 43:6239

    Article  CAS  Google Scholar 

  • Kozlik-Feldmann R, Lang N, Aumann R, Lehner A, Rassoulian D, Sodian R, Schmitz C, Hinterseer M, Hinkel R, Thein E, Freudenthal F, Vasilyev NV, Nido PJ del, Netz H (2008) Patch closure of muscular ventricular septal defects with a new hybrid therapy in a pig model. J Am Coll Cardiol 51:1597–1603

    Article  PubMed  Google Scholar 

  • Kulkeaw K, Horio Y, Mizuochi C, Ogawa M, Sugiyama D (2010) Variation in hematopoietic potential of induced pluripotent stem cell lines. Stem Cell Rev (in press)

  • Lutolf MP, Gilbert PM, Blau HM (2009) Designing materials to direct stem-cell fate. Nature 462:433–441

    Article  CAS  PubMed  Google Scholar 

  • Monaco E, Lima A, Bionaz M, Maki A, Wilson MS, Hurley LW, Wheeler MB (2009) Morphological and transcriptomic comparison of adipose and bone marrow derived porcine stem cells. Open Tissue Eng Regen Med J 2:20–33

    Article  CAS  Google Scholar 

  • Patrick CW Jr (2000) Adipose tissue engineering: the future of breast and soft tissue reconstruction following tumor resection. Semin Surg Oncol 19:302–311

    Article  PubMed  Google Scholar 

  • Patrick CW Jr (2001) Tissue engineering strategies for adipose tissue repair. Anat Rec 263:361–366

    Article  CAS  PubMed  Google Scholar 

  • Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147

    Article  CAS  PubMed  Google Scholar 

  • Talens-Visconti R, Bonora A, Jover R, Mirabet V, Carbonell F, Castell JV, Gomez-Lechon MJ (2006) Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells. World J Gastroenterol 12:5834–5845

    CAS  PubMed  Google Scholar 

  • Tavakoli T, Xu X, Derby E, Serebryakova Y, Reid Y, Rao MS, Mattson MP, Ma W (2009) Self-renewal and differentiation capabilities are variable between human embryonic stem cell lines I3, I6 and BG01V. BMC Cell Biol 10:44

    Article  PubMed  Google Scholar 

  • Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U, Krause U, Blake J, Schwager C, Eckstein V, Ansorge W, Ho AD (2005) Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 33:1402–1416

    Article  CAS  PubMed  Google Scholar 

  • Walker MR, Patel KK, Stappenbeck TS (2009) The stem cell niche. J Pathol 217:169–180

    Article  CAS  PubMed  Google Scholar 

  • Watt FM, Hogan BL (2000) Out of Eden: stem cells and their niches. Science 287:1427–1430

    Article  CAS  PubMed  Google Scholar 

  • Weissman IL (2000) Translating stem and progenitor cell biology to the clinic: barriers and opportunities. Science 287:1442–1446

    Article  CAS  PubMed  Google Scholar 

  • Zhu YX, Liu TQ, Song KD, Fan XB, Ma XH, Cui ZF (2008) Adipose-derived stem cell: a better stem cell than BMSC. Cell Res 18:1

    Article  Google Scholar 

  • Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228

    Article  CAS  PubMed  Google Scholar 

  • Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Stem Cell Biology and Engineering, Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Ill., USA

    Dongshin Kim, Elisa Monaco, Aaron Maki, Alecsandra Sobreira de Lima, Walter L. Hurley & Matthew B. Wheeler

  2. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Ill., USA

    Dongshin Kim, Elisa Monaco, Aaron Maki, Hyun Joon Kong & Matthew B. Wheeler

  3. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Ill., USA

    Hyun Joon Kong

Authors
  1. Dongshin Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elisa Monaco
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aaron Maki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alecsandra Sobreira de Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hyun Joon Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Walter L. Hurley
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Matthew B. Wheeler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Matthew B. Wheeler.

Additional information

Dongshin Kim and Elisa Monaco contributed equally to this work.

This work was supported by a grant from the Illinois Regenerative Medicine Institute (IDPH 63080017).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, D., Monaco, E., Maki, A. et al. Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels. Cell Tissue Res 341, 359–370 (2010). https://doi.org/10.1007/s00441-010-1015-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00441-010-1015-3

Keywords

Search

Navigation