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Discrete localization of various fatty-acid-binding proteins in various cell populations of mouse retina

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Abstract

Various fatty acids (FAs) are involved as an energy source in many different functions in the organism. They are also essential ingredients of membranous lipids and act as intracellular signaling molecules. Intracellular fatty-acid-binding proteins (FABPs) comprise a family of soluble lipid-binding proteins with low molecular masses and solubilize long-chain FAs to allow intracellular translocation in the aqueous cytosol. To clarify the functions of FABPs in the retina, which is remarkably rich in polyunsaturated FAs, we have investigated the localization of B (brain type)-, H (heart type)-, E (epidermal type)-, and A (adipocyte type)-FABPs in adult mouse retinae by immunohistochemistry. In order to determine the possible involvement of FABPs in retinal degenerative diseases, we have also examined changes in FABP expression in light-induced photoreceptor cell degeneration (photic injury). The discrete localization of B-, H-, E-, and A-FABP species in various cell populations of the retina has been clarified: B-FABP is mainly localized in the cone photoreceptor cells, H-FABP in some populations of amacrine/bipolar/horizontal interneurons, and E-FABP in ganglion cells, with A-FABP-like immunoreactivity being located in resident microglia of normal retinae. E-FABP has further been localized in invasive macrophages in damaged retinae following photic injury, allowing discrete identification of the resident microglia and invasive macrophages by A- and E-FABP immunoreactivity, respectively.

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Authors and Affiliations

  1. Division of Histology, Department of Cell Biology, Tohoku University Graduate School of Medical Sciences, 980-8575, Sendai, Japan

    Sachiko Saino-Saito, Reza Mohammad Nourani, Hiroo Iwasa & Hisatake Kondo

  2. Department of Cell Biology, Chemical Injuries Research Center, Baqiyatallah Medical Sciences University, 14359-16471, Tehran, Iran

    Reza Mohammad Nourani

  3. Division of Histology, Department of Rehabilitation, Faculty of Health Science and Welfare, Tohoku Bunka Gakuen University, 6-45-1 Kunimi, 981-8551, Sendai, Japan

    Sachiko Saino-Saito & Hisatake Kondo

  4. Department of Anatomy, Yamaguchi University School of Medicine, 755-8505, Ube, Japan

    Yuji Owada

  5. Yamagata Prefectural Kahoku Hospital, 999-3511, Kahoku, Japan

    Sachiko Saino-Saito

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  1. Sachiko Saino-Saito
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  2. Reza Mohammad Nourani
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  3. Hiroo Iwasa
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  4. Hisatake Kondo
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  5. Yuji Owada
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Correspondence to Hisatake Kondo.

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This study was supported by grant nos. 20590180 and 18390056 from the Ministry of Education, Science, and Culture of Japan to S.S and H.K., respectively.

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Saino-Saito, S., Nourani, R.M., Iwasa, H. et al. Discrete localization of various fatty-acid-binding proteins in various cell populations of mouse retina. Cell Tissue Res 338, 191–201 (2009). https://doi.org/10.1007/s00441-009-0862-2

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  • DOI: https://doi.org/10.1007/s00441-009-0862-2

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