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Expression and localization of growth hormone and its receptors in the chicken ovary during sexual maturation

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Abstract

Roles of pituitary growth hormone (GH) in female reproduction are well established. Autocrine and/or paracrine actions of GH in the mammalian ovary have additionally been proposed, although whether the ovary is an extra-pituitary site of GH expression in the laying hen is uncertain. This possibility has therefore been assessed in the ovaries of Hy-Line hens before (between 10–16 weeks of age) and after (week 17) the onset of egg laying. Reverse transcription/polymerase chain reaction (RT-PCR) analysis has consistently detected a full-length (690 bp) pituitary GH cDNA in ovarian stroma from 10 weeks of age, although GH expression is far lower than that in the pituitary gland or hypothalamus. GH mRNA is also present in small (>1–4 mm diameter) follicles after their ontogenetic appearance at 14 weeks of age and in all other developing follicles after 16 weeks of age (>4–30 mm diameter). Immunoreactivity for GH is similarly present in the ovarian stroma from 10 weeks of age and in small (<4 mm diameter) and large (>4–30 mm) follicles from 14 and 16 weeks of age, respectively. The relative intensity of GH staining in the ovarian follicles is consistently greater in the granulosa cells than in the thecal cells and is comparable with that in the follicular epithelium. A 321-bp fragment of GH receptor (GHR) cDNA, coding for the intracellular domain of the receptor, has also been detected by RT-PCR in the ovary and is present in stromal tissue by 10 weeks of age, in small follicles (<4 mm diameter) by 14 weeks of age, and in larger follicles (>4–30 mm diameter) from 16 weeks. GHR immunoreactivity has similarly been detected, like GH, in the developing ovary and in all follicles and is more intense in granulosa cells than in the theca interna or externa. The expression and location of the GH gene therefore parallels that of the GHR gene during ovarian development in the laying hen, as does the appearance of GH and GHR immunoreactivity. These results support the possibility that GH has autocrine and/or paracrine actions in ovarian function prior to and after the onset of lay in hens.

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References

  • Abir R, Garor R, Felz C, Nitke S, Krissi H, Fisch B (2007) Growth hormone and its receptor in human ovaries from fetuses and adults. Fertil Steril DOI 10.1016/j.fertnstert.2007.08.011

  • Ahmed S, Harvey S (2002) Ghrelin: a hypothalamic GH-releasing factor in domestic fowl (Gallus domesticus). J Endocrinol 172:117–125

    Article  PubMed  CAS  Google Scholar 

  • Apa R, Lanzone A, Miceli F, Mastrandrea M, Caruso A, Mancuso S, Canipari R (1994) Growth hormone induces in vitro maturation of follicle and cumulus-enclosed rat oocytes. Mol Cell Endocrinol 106:207–212

    Article  PubMed  CAS  Google Scholar 

  • Arámburo C, Carranza M, Sanchez R, Perera G (1989) Partial biochemical and biological characterization of purified chicken growth hormone (cGH). Isolation of cGH change variants and evidence that cGH is phosphorylated. Gen Comp Endocrinol 76:330–339

    Article  PubMed  Google Scholar 

  • Arámburo C, Navarrete S, Montiel JL, Sanchez R, Berghman LR (1991) Purification and electrophoretic analysis of glycosylated chicken growth hormone G-cGH: evidence of G-cGH isoforms. Gen Com Endocrinol 84:135–146

    Article  Google Scholar 

  • Arámburo C, Luna M, Carranza M, Reyes M, Martinez-Caria H, Scanes CG (2000) Growth hormone size variants: changes in the pituitary during development of the chicken. Proc Soc Exp Biol Med 223:67–74

    Article  PubMed  Google Scholar 

  • Arámburo C, Carranza M, Martinez-Caria H, Reyes M, Berumen L, Lopez J, Pascacio H, Huerta L, Luna M (2001) Molecular and functional heterogeneity of growth hormone. In: Dawson A, Chaturvedi CM (eds) Avian endocrinology. Narosa, New Dehli Chennai Mumbai Kolkata, pp 273–286

    Google Scholar 

  • Bachelot A, Monget P, Imbert-Bolloré P, Coshigano K, Kopchick JJ, Kelly PA, Binart N (2002) Growth hormone is required for ovarian follicular growth. Endocrinology 143:4104–4112

    Article  PubMed  CAS  Google Scholar 

  • Baudet ML, Harvey S (2003) Ghrelin-induced GH secretion in domestic fowl in vivo and in vitro. J Endocrinol 179:97–105

    Article  PubMed  CAS  Google Scholar 

  • Baumann G (1991) Growth hormone heterogeneity: genes, isohormones, variants, and binding proteins. Endocrine Rev 12:424–447

    CAS  Google Scholar 

  • Bedrak E, Harvey S, Chadwick A (1981) Concentrations of pituitary, gonadal and adrenal hormones in serum of laying and broody white rock hens (Gallus domesticus). J Endocrinol 89:197–204

    PubMed  CAS  Google Scholar 

  • Berghman LR, Beeumen J van, Decuypere E, Kuhn ER, Vandesande F (1988) One-step purification of chicken growth hormone from a crude pituitary extract by use of a monoclonal immunoadsorbent. J Endocrinol 118:381–387

    Google Scholar 

  • Bossis I, Porter TE (2001) Identification of the somatostatin receptor subtypes involved in regulation of growth hormone secretion in chickens. Mol Cell Endocrinol 182:203–213

    Article  PubMed  CAS  Google Scholar 

  • Bruggeman V, D’Hondt E, Berghman L, Onagbesan O, Vanmontfort D, Vandesande F, Decuypere E (1998) The effect of food intake from 2 to 24 weeks of age on LHRH-I content in the median eminence and gonadotrophin levels in pituitary and plasma in female broiler breeder chickens. Gen Comp Endocrinol 112:200–209

    Article  PubMed  CAS  Google Scholar 

  • Burnside J, Liou SS, Cogburn LA (1991) Molecular cloning of the chicken growth hormone receptor complementary deoxyribonucleic acid: mutation of the gene in sex-linked dwarf chickens. Endocrinology 128:3183–3192

    Article  PubMed  CAS  Google Scholar 

  • Cochran RA, Leonardi-Cattolica AA, Sullivan MR, Kincaid LA, Leise BS, Thompson DL, Godke RA (1999) The effect of equine somatotropin (eST) on follicular development and circulating plasma hormone profiles in cyclic mares treated during different stage of the estrous cycle. Domest Anim Endocrinol 16:57–67

    Article  PubMed  CAS  Google Scholar 

  • Codner E, Cassorla F (2002) Growth hormone and reproductive function. Mol Cell Endocrinol 186:133–136

    Article  CAS  Google Scholar 

  • De laSota RL, Lucy MC, Staples CR, Thatcher WW (1993) Effects of recombinant bovine somatotropin on ovarian function in lactating and nonlactating dairy cows. J Dairy Sci 76:1002–1013

    Article  Google Scholar 

  • Donoghue DJ, Campbell RM, Scanes CG (1990) Effect of biosynthetic chicken growth hormone on egg production in White Leghorn hens. Poult Sci 69:1818–1821

    PubMed  CAS  Google Scholar 

  • Filby AL, Tyler CR (2007) Cloning and characterization of cDNAs for hormones and/or receptors of growth hormone, insulin-like growth factor-I, thyroid hormone, and corticosteroid and the gender-, tissue-, and developmental-specific expression of their mRNA transcripts in fathead minnow (Pimephales promelas). Gen Comp Endocrinol 150:151–163

    Article  PubMed  CAS  Google Scholar 

  • Gong JG, Bramley TA, Webb R (1993) The effect of recombinant bovine somatotrophin on ovarian follicular growth and development in heifer. J Reprod Fertil 97:247–254

    Article  PubMed  CAS  Google Scholar 

  • Grzegorzewska A, Paczoska-Eliasiewicz HE (2006) Expression of leptin receptors mRNA in hypothalamus, pituitary and gonads in chicken embryos. Acta Biol Cracov Ser Zool 48:67–73

    Google Scholar 

  • Hagen DR, Graboski RA (1990) Effect of porcine pituitary growth hormone (pGH) on cytoplasmic maturation of porcine oocytes in vitro. J Anim Sci 68:446

    Google Scholar 

  • Harvey S (1990) Thyrotropin releasing hormone: a growth hormone releasing hormone. J Endocrinol 125:345–358

    PubMed  CAS  Google Scholar 

  • Harvey S, Scanes CG, Chadwick A, Bolton NJ (1978) The effect of thyrotropin-releasing hormone (TRH) and somatostatin (GHRIH) on growth hormone and prolactin secretion in vitro and in vivo in the domestic fowl (Gallus domesticus). Neuroendocrinology 26:249–260

    Article  PubMed  CAS  Google Scholar 

  • Harvey S, Scanes CG, Chadwick A, Bolton NJ (1979) Growth hormone and prolactin secretion in growing domestic fowl: influence of sex and breed. Br Poult Sci 20:9–17

    Article  PubMed  CAS  Google Scholar 

  • Harvey S, Johnson CDM, Sanders EJ (2000) Extra-pituitary growth hormone in peripheral tissues of early chick embryos. J Endocrinol 166:489–502

    Article  PubMed  CAS  Google Scholar 

  • Harvey S, Baudet ML, Murphy A, Luna M, Hull KL, Arámburo C (2004) Testicular growth hormone (GH): GH expression in spermatogonia and primary spermatocytes. Gen Comp Endorinol 139:158–167

    Article  CAS  Google Scholar 

  • Heck A, Matayer S, Onagbesan OM, Williams J (2003) mRNA expression of components of the IGF system and of GH and insulin receptors in ovaries of broiler breeder hens fed ad libitum or restricted from 4 to 16 weeks of age. Domest Anim Endocrinol 25:287–294

    Article  PubMed  CAS  Google Scholar 

  • Hrabia A, Rząsa J (2007) Effect of growth hormone administration on steroids level in chicken ovary during sexual maturation. 16th Intern. Symposium of Polish Network of Molecular and Cellular Biology, UNESCO/PAS: Molecular and Physiological Aspects of Regulatory Processes of the Organism, Cracow, pp 161–162

  • Hrabia A, Rząsa J, Paczoska-Eliasiewicz H (2001) The effect of GH on steroid hormones secretion by isolated chicken ovarian follicles. 10th Intern. Symposium of Polish Network of Molecular and Cellular Biology, UNESCO/PAS: Molecular and Physiological Aspects of Regulatory Processes of the Organism, Cracow, pp 134–135

  • Hull KL, Harvey S (2000) Growth hormone: a reproductive endocrine-paracrine regulator? Rev Reprod 5:175–182

    Article  PubMed  CAS  Google Scholar 

  • Hull KL, Harvey S (2001) Growth hormone: roles in female reproduction. J Endocrinol 168:1–23

    Article  PubMed  CAS  Google Scholar 

  • Hull KL, Thiagarajah A, Harvey S (1996) Cellular localisation of growth hormone receptors/binding proteins in immune tissues. Cell Tissue Res 286:69–80

    Article  PubMed  CAS  Google Scholar 

  • Izadyar F, Colenbrander B, Bevers MM (1996) In vitro maturation of bovine oocytes in the presence of growth hormone accelerates nuclear maturation and promotes subsequent embryonic development. Mol Reprod Dev 45:372–378

    Article  PubMed  CAS  Google Scholar 

  • Izadyar F, Zhao J, Tol HTA von, Colenbrander B, Bevers MM (1999) Messenger RNA expression and protein localization of growth hormone in bovine ovarian tissue and in cumulus oocyte complexes (COCs) during in vitro maturation. Mol Reprod Dev 53:398–406

    Article  PubMed  CAS  Google Scholar 

  • Kelly PA, Ali S, Rozakis M, Goujon L, Nagano M, Pellegrini I, Gould D, Djiane J, Edery M, Finidori J, Postel-Vinay MC (1993) The growth hormone/prolactin receptor family. Recent Prog Horm Res 48:123–164

    PubMed  CAS  Google Scholar 

  • Kobayashi J, Mizunuma H, Kikuchi N, Liu X, Andoh K, Abe Y, Yokota H, Yamada K, Ibuki Y, Hagiwara H (2000) Morphological assessment of the effect of growth hormone on preantral follicles from 11-day-old mice in an in vitro culture system. Biochem Biophys Res Commun 268:36–41

    Article  PubMed  CAS  Google Scholar 

  • Kölle S, Stojkovic M, Boie G, Wolf E, Sinowatz F (2003) Growth hormone-related effects on apoptosis, mitosis, and expression of connexin 43 in bovine in vitro maturation cumulus-oocyte complexes. Biol Reprod 68:1584–1589

    Article  PubMed  CAS  Google Scholar 

  • Lamb IC, Galehouse DM, Foster DN (1988) Chicken growth hormone cDNA sequence. Nucleic Acids Res 16:9339

    Article  PubMed  CAS  Google Scholar 

  • Lebedeva IY, Lebedev VA, Grossmann R, Kuzmina TI, Parvizi N (2004) Characterization of growth hormone binding sites in granulosa and theca layers at different stage of follicular maturation and ovulatory cycle in the domestic hen. Biol Reprod 71:1174–1181

    Article  PubMed  CAS  Google Scholar 

  • Lee LTO, Siu FKY, Tam JKV, Lau ITY, Wong AOL, Lin MCM, Vaudry H, Chow KC (2007) Discovery of growth hormone-releasing hormones and receptors in nonmammalian vertebrates. Proc Natl Acad Sci USA 104:2133–2138

    Article  PubMed  CAS  Google Scholar 

  • Leung DW, Spencer SA, Cachianes G, Hammonds RG, Collins C, Henzel WJ, Bernard R, Waters MJ, Wood WI (1987) Growth hormone receptor and serum binding protein purification, cloning and expression. Nature 330:537–543

    Article  PubMed  CAS  Google Scholar 

  • Lewis UJ (1992) Growth hormone: what is it, what does it do? Trends Endocrinol Metab 3:117–121

    Article  CAS  PubMed  Google Scholar 

  • Li WS, Chen D, Wong AO, Lin HR (2005) Molecular cloning, tissue distribution, and ontogeny of mRNA expression of growth hormone in orange-spotted grouper (Epinephelus coioides). Gen Comp Endocrinol 144:78–89

    Article  PubMed  CAS  Google Scholar 

  • Lovell TM, Vanmonfort D, Bruggeman V, Decuypere E, Groome NP, Knight PG, Gladwel RT (2000) Circulating concentrations of inhibin-related proteins during the ovulatory cycle of the domestic fowl (Gallus domesticus) and after induced cessation of egg laying. J Reprod Fertil 119:323–328

    Article  PubMed  CAS  Google Scholar 

  • Lovell TM, Gladwell RT, Groome NP, Knight PG (2002) Modulatory effects of gonadotrophins and insulin-like growth factor on the secretion of inhibin A and progesterone by granulosa cells from chicken preovulatory (F1-F3) follicles. Reproduction 123:291–300

    Article  PubMed  CAS  Google Scholar 

  • Luna M, Huerta L, Berumen L, Martinez-Coria H, Harvey S, Arámburo C (2004) Growth hormone in the male reproductive tract of the chicken: heterogeneity and changes during ontogeny and maturation. Gen Comp Endocrinol 137:37–49

    Article  PubMed  CAS  Google Scholar 

  • Onagbesan OM, Vleugels B, Buys N, Bruggeman V, Safi M, Decuypere E (1999) Insulin-like growth factors in the regulation of avian ovarian functions. Domest Anim Endocrinol 17:299–313

    Article  PubMed  CAS  Google Scholar 

  • Onagbesan OM, Mast J, Goddeeris B, Decuypere E (2000) Effect of TNF-α on LH and IGF-I modulated chicken granulosa cell proliferation and progesterone production during follicular development. J Reprod Fertil 120:433–442

    Article  PubMed  CAS  Google Scholar 

  • Ozawa K, Mizunuma H, Ozawa H, Ibuki Y (1996) Recombinant human growth hormone acts on intermediate-sized follicles and rescues growing follicles from atresia. Endocr J 43:87–92

    Article  PubMed  CAS  Google Scholar 

  • Paczoska-Eliasiewicz HE, Salomon G, Reicher S, Gussakowsky E, Hrabia A, Gertler A (2006) Preparation and characterization of recombinant chicken growth hormone (chGH) and putative antagonist chGH G119R mutein. Ann N Y Acad Sci 1091:501–508

    Article  PubMed  CAS  Google Scholar 

  • Pilecka I, Whatmore A, Huijsduijnen RH, Destenaves B, Clayton P (2007) Growth hormone signaling: sprouting links between pathways, human genetics and therapeutic options. Trends Endocrinol Metab 18:12–18

    Article  PubMed  CAS  Google Scholar 

  • Piper MM, Porter TE (1997) Responsiveness of chicken embryonic somatotropes to somatostatin (SRIF) and IGF-I. J Endocrinol 154:303–310

    Article  PubMed  CAS  Google Scholar 

  • Porter TE, Ellestad LE, Fay A, Steward JL, Bossis I (2006) Identification of the chicken growth hormone-releasing hormone receptor (GHRH-R) mRNA and gene: regulation of anterior pituitary GHRH-R mRNA levels by homologous and heterologous hormones. Endocrinology 147:2535–2543

    Article  PubMed  CAS  Google Scholar 

  • Rousseau K, Dufour S (2007) Comparative aspects of GH and metabolic regulation in lower vertebrates. Neuroendocrinology 86:165–174

    Article  PubMed  CAS  Google Scholar 

  • Rząsa J, Mika M, Paczoska-Eliasiewicz H (1997) Changes of steroid hormones in chicken ovarian follicles during sexual maturation (in Polish). Zeszt Nauk Przeg Hod 31:101–104

    Google Scholar 

  • Scanes CG, Harvey S (1982) Hormones, nutrition, and metabolism in birds. In: Scanes CG (ed) Aspects of avian endocrinology: practical and theoretical implications, vol 26. Grad Studies, Texas Technical University, Lubbock, pp 1–411

    Google Scholar 

  • Scanes CG, Griminger P (1990) Endocrine-nutrition interactions in birds. J Exp Zool Suppl 4:98–105

    Article  PubMed  CAS  Google Scholar 

  • Scanes CG, Sharp PJ, Harvey S, Godden PM, Chadwick A, Newcomer WS (1979) Variations in plasma prolactin, thyroid hormones, gonadal steroids and growth hormone in turkeys during the induction of egg laying and moult by different photoperiods. Br Poult Sci 20:143–148

    Article  PubMed  CAS  Google Scholar 

  • Schwarzler P, Untergasser G, Hermann M, Dirnhofer S, Abendstein B, Madersbacher S, Berger P (1997) Selective growth hormone/placental lactogen gene transcription and hormones production in pre- and postmenopausal human ovaries. J Clin Endocrinol Metab 82:3337–3341

    Article  PubMed  CAS  Google Scholar 

  • Sharp PJ, Scanes CG, Williams JB, Harvey S, Chadwick A (1979) Variations in concentrations of prolactin, luteinizing hormone, growth hormone and progesterone in the plasma of broody bantams (Gallus domesticus). J Endocrinol 80:51–57

    Article  PubMed  CAS  Google Scholar 

  • Shimizu T, Murayama Ch, Sudo N, Kawashima Ch, Tetsuka M, Miyamoto A (2007) Involvement of insulin and growth hormone (GH) during follicular development in the bovine ovary. Anim Reprod Sci DOI 10.1016/j.anireprosci.2007.04.005

  • Sirotkin AV, Grossmann R (2003) Role of tyrosine kinase- and MAP kinase-dependent intracellular mechanisms in control of ovarian functions in the domestic fowl (Gallus domesticus) and in mediating effects of IGF-II. J Reprod Dev 49:99–106

    Article  PubMed  CAS  Google Scholar 

  • Sirotkin AV, Grossmann R (2007) The role of ghrelin and some intracellular mechanisms in controlling the secretory activity of chicken ovarian cells. Comp Biochem Physiol [A] 147:239–246

    CAS  Google Scholar 

  • Sirotkin AV, Grossmann R, Maria-Peon MT, Roa J, Tena-Sempere M, Klein S (2006) Novel expression and functional role of ghrelin in chicken ovary. Mol Cell Endocrinol 257–258:15–25

    Article  PubMed  CAS  Google Scholar 

  • Spencer GS, Buyse J, Decuypere E, Rahimi G (1997) Physiological inhibition of growth hormone secretion by both insulin-like growth factors-I and -II in chickens. Br Poult Sci 38:429–431

    Article  PubMed  CAS  Google Scholar 

  • Sun JM, Richards MP, Rosebrough RW, Ashwell CM, McMurtry JP, Coon CN (2006) The relationship of body composition, feed intake, and metabolic hormones for broiler breeder females. Poult Sci 85:1173–1184

    PubMed  CAS  Google Scholar 

  • Tanabe Y, Ogawa T, Nakamura T (1981) The effect of short term starvation on pituitary and plasma LH, plasma estradiol and progesterone, and on pituitary response to LH-RH in the laying hens (Gallus domesticus). Gen Comp Endocrinol 43:392–398

    Article  PubMed  CAS  Google Scholar 

  • Tanaka M, Yamamoto I, Ohkubo T, Wakita M, Hoshino S, Nakashima K (1999) cDNA cloning and developmental alterations in gene expression of the two Pit-1/GHF-1 transcription factors in the chicken pituitary. Gen Comp Endocrinol 114:441–448

    Article  PubMed  CAS  Google Scholar 

  • van Tuinen M, Sibley CG, Hedges SB (2000) The early history of modern birds inferred from DNA sequences of nuclear and mitochondrial ribosomal genes. Mol Biol Evol 17:451–457

    Google Scholar 

  • Van As P, Buys N, Onagbesan OM, Decuypere E (2000) Complementary DNA cloning and ontogenic expression of pituitary-specific transcription factor of chickens (Gallus domesticus) from the pituitary gland. Gen Comp Endocrinol 120:127–136

    Article  PubMed  CAS  Google Scholar 

  • Van As P, Janssens K, Pals K, De Groef B, Onagbesan OM, Bruggeman V, Darras VM, Denef C, Decuypere E (2006) The chicken pituitary-specific transcription factor PIT-1 is involved in the hypothalamic regulation of pituitary hormones. Acta Vet Hung 54:455–471

    Article  PubMed  CAS  Google Scholar 

  • Vanmonfort D, Berghman LR, Rombauts L, Verhoeven G, Decuypere E (1994) Changes of immunoreactive inhibin, follicle-stimulating hormone, luteinizing hormone, and progesterone in plasma after short-term food deprivation and during the ovulatory cycle of the domestic hen. Gen Comp Endocrinol 95:117–124

    Article  Google Scholar 

  • Vos AM de, Ultsch M, Kossiakoff AA (1992) Human growth hormone and extracellular domain of its receptor: crystal structure of complex. Science 255:306–312

    Article  PubMed  Google Scholar 

  • Wang Y, Li J, Wang CY, Kwok AHY, Leung FC (2007) Identification of the endogenous ligand for chicken growth hormone-releasing hormone (GHRH) receptor: evidence for a separate gene encoding GHRH in submammalian vertebrates. Endocrinology 148:2405–2416

    Article  PubMed  CAS  Google Scholar 

  • Williams J, Harvey S, Leclercq B (1986) Plasma levels of luteinizing hormone, growth hormone, and estradiol from six weeks of age to sexual maturity in two lines of chickens selected for low or high abdominal fat content. Poult Sci 65:1782–1786

    PubMed  CAS  Google Scholar 

  • Williams J, Sharp PJ, Goddard C (1992) The effect of growth hormone on ovarian follicular growth in the domestic hen. J Reprod Fertil (Abstr) Series 9–10:59

    Google Scholar 

  • Yang BY, Greene M, Chen TT (1999) Early embryonic expression of the growth hormone family protein genes in the developing rainbow trout, Oncorhynchus mykiss. Mol Reprod Dev 53:127–134

    Article  PubMed  CAS  Google Scholar 

  • Yoshimura Y, Nakamura Y, Koyama N (1993) Effect of growth hormone on follicle growth, oocyte maturation, and ovarian steroidogenesis. Fertil Steril 59:917–923

    PubMed  CAS  Google Scholar 

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Acknowledgements

The authors thank Ms. Grażyna Dąbrowska, Ms. Maria Ogórek, and Ms. Barbara Witkowska from the Agricultural University of Krakow for technical help during experiments and Dr. Agnieszka Grzegorzewska for help with the RT-PCR analysis.

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Authors and Affiliations

  1. Department of Animal Physiology, Agricultural University in Krakow, Al. Mickiewicza 24/28, 30-059, Krakow, Poland

    Anna Hrabia, Helena E. Paczoska-Eliasiewicz & Janusz Rząsa

  2. Department of Poultry Science and Veterinary Pathobiology, Texas A&M University, College Station, Tex., USA

    Luc R. Berghman

  3. Department of Physiology, University of Alberta, Edmonton, AB, Canada

    Steve Harvey

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  1. Anna Hrabia
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  2. Helena E. Paczoska-Eliasiewicz
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  3. Luc R. Berghman
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  4. Steve Harvey
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Correspondence to Anna Hrabia.

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This study was supported by grant no. 2 P06D 034 28 from the Ministry of Education and Science, Poland (to A.H.) and, in part, by a Discovery Grant from NSERC (to S.H.).

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Hrabia, A., Paczoska-Eliasiewicz, H.E., Berghman, L.R. et al. Expression and localization of growth hormone and its receptors in the chicken ovary during sexual maturation. Cell Tissue Res 332, 317–328 (2008). https://doi.org/10.1007/s00441-008-0595-7

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