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Immunolocalisation of crustacean-SIFamide in the median brain and eyestalk neuropils of the marbled crayfish

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Abstract

Crustacean-SIFamide (GYRKPPFNGSIFamide) is a novel neuropeptide that was recently isolated from crayfish nervous tissue. We mapped the localisation of this peptide in the median brain and eyestalk neuropils of the marbled crayfish (Marmorkrebs), a parthenogenetic crustacean. Our experiments showed that crustacean-SIFamide is strongly expressed in all major compartments of the crayfish brain, including all three optic neuropils, the lateral protocerebrum with the hemiellipsoid body, and the medial protocerebrum with the central complex. These findings imply a role of this peptide in visual processing already at the level of the lamina but also at the level of the deeper relay stations. Immunolabelling is particularly strong in the accessory lobes and the deutocerebral olfactory lobes that receive a chemosensory input from the first antennae. Most cells of the olfactory globular tract, a projection neuron pathway that links deuto- and protocerebrum, are labelled. This pathway plays a central role in conveying tactile and olfactory stimuli to the lateral protocerebrum, where this input converges with optic information. Weak labelling is also present in the tritocerebrum that is associated with the mechanosensory second antennae. Taken together, we suggest an important role of crustacean-SIFamidergic neurons in processing high-order, multimodal input in the crayfish brain.

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Authors and Affiliations

  1. Institute of Infectious Diseases, Medical University of Warsaw, Warsaw, 02-106, Poland

    M. A. Polanska

  2. Suntory Institute for Bioorganic Research, Wakayamadai, Shimamoto, Mishima, Osaka, 618-8503, Japan

    A. Yasuda

  3. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, Hans-Knöll-Str. 8, 07745, Jena, Germany

    S. Harzsch

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  1. M. A. Polanska
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  2. A. Yasuda
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  3. S. Harzsch
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Correspondence to S. Harzsch.

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Harald Wolf is gratefully acknowledged for providing laboratory facilities at the University of Ulm, and Kathia Fabritius-Vilpoux and Verena Rieger for assistance with rearing the crayfish. We wish to thank David Sandeman and Barbara Beltz for discussion on the crayfish brain architecture and comments on an earlier version of this manuscript. Our special thanks go to Andy Sombke for his assistance in assembling the final figures. This study was supported by DFG grants Ha 2540/3 and 6.

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Polanska, M.A., Yasuda, A. & Harzsch, S. Immunolocalisation of crustacean-SIFamide in the median brain and eyestalk neuropils of the marbled crayfish. Cell Tissue Res 330, 331–344 (2007). https://doi.org/10.1007/s00441-007-0473-8

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