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Abnormal recruitment of periendothelial cells to lymphatic capillaries in digestive organs of angiopoietin-2-deficient mice

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Abstract

The fine structure of lymphatic capillaries in the digestive organs of angiopoietin-2 (Ang2) knockout mice was studied by using both immunohistochemistry and electron microscopy. The genetic deletion of Ang2 yielded hypoplasia and disorganization of the lymphatic capillaries, with their shapes being irregular, and an aberrant recruitment of vascular periendothelial cells immunopositive for smooth muscle actin to the lymphatic capillaries. The abnormal lymphatic periendothelial cells were considered to be a type of pericyte for the lymphatic capillaries after the deletion of Ang2, because they were ultrastructurally characterized by abundant thin myofilaments in their cytoplasm and long cytoplasmic extensions similar to those shown by blood vascular pericytes. The genetic replacement of Ang2 with Ang1 rescued the defects, viz., the disorganization and disordered structure of the lymphatic capillaries. The present findings suggest that Ang2 serves the morphogenesis of lymphatic capillaries as an agonist for the receptor, Tie2, and that Ang1 can replace Ang2 in guiding lymphatic formation and development.

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Authors and Affiliations

  1. Department of Surgery, The University of Arizona, Tucson, AZ, USA

    Hiroshi Shimoda, Michael J. Bernas & Marlys H. Witte

  2. Regeneron Pharmaceuticals, Tarrytown, NY, USA

    Nicholas W. Gale & George D. Yancopoulos

  3. Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Oita, 879-5593, Japan

    Hiroshi Shimoda & Seiji Kato

Authors
  1. Hiroshi Shimoda
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  2. Michael J. Bernas
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  3. Marlys H. Witte
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  4. Nicholas W. Gale
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  5. George D. Yancopoulos
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  6. Seiji Kato
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Corresponding author

Correspondence to Hiroshi Shimoda.

Additional information

H. Shimoda and M. Witte were supported by an Exchange Visitor Program Grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by Contract 6011 from the Arizona Disease Control Research Commission, respectively.

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Shimoda, H., Bernas, M.J., Witte, M.H. et al. Abnormal recruitment of periendothelial cells to lymphatic capillaries in digestive organs of angiopoietin-2-deficient mice. Cell Tissue Res 328, 329–337 (2007). https://doi.org/10.1007/s00441-006-0360-8

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  • DOI: https://doi.org/10.1007/s00441-006-0360-8

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