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Origin of follicular dendritic cell in the chicken spleen

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Abstract

The ellipsoid-associated cell (EAC) is a blood-borne phagocytic cell, residing in the antigen trapping zone of the chicken spleen. Binding and endocytosis of βGalactosidase (βGal) are independent from the Fc and complement receptors, because sulfated polysaccharides, in a concentration manner, inhibit the bacterial antigen uptake. The βGal-positive cells migrate to the periarterial lymphatic sheath (PALS), the preexisting germinal centers (GC), and form clusters with B- and T-cells. βGal, E5G12 double positive cells on the surface of the ellipsoid and in the PALS, GC and clusters prove that the EACs carry the enzyme. The EAC and the follicular dendritic cell (FDC) express, 68.2 and E5G12 and, 74.3 and E5G12, antigens, respectively. During migration the cessation of 68.2 and expression of 74.3 indicate the differentiation of EAC to FDC. By day 14 the clusters had disappeared, and in several GC the presence of double positive cells (74.3 and βGal; E5G12 and βGal) showed that the clusters had developed to GC. The presence of βGal+ cells in the PALS, where interdigitating dendritic cells (IDC) cooperate with the T-cells, suggests that in the spleen alternate routes exist for the EAC differentiation to FDC: EAC to FDC: βGal-loaded cells in the preexisting GC; and EAC through IDC to FDC: βGal+ EAC in the PALS and clusters. The EAC-FDC axis works exclusively inside the spleen; therefore; this system may be operated in pneumococcus infection.

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Abbreviations

EAC:

Ellipsoid-associated cell

PALS:

Periarterial lymphatic sheath

PWP:

Periellipsoidal white pulp

GC:

Germinal center

FDC:

Follicular dendritic cell

IDC:

Interdigitating dendritic cell

βGal:

βGalactosidase

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Acknowledgements

The authors wish to thank to Zsuzsanna Vidra and Beáta Urák for the laboratory and photographical assistance, and appreciate the editing done by Scott D. Everett.

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Authors and Affiliations

  1. Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, H-1094, Tűzoltó u. 58., Budapest, Hungary

    Botond-Zoltán Igyártó, Attila Magyar & Imre Oláh

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  1. Botond-Zoltán Igyártó
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  2. Attila Magyar
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  3. Imre Oláh
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Correspondence to Imre Oláh.

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This work was supported by OTKA Grant number: T-042558.

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Igyártó, BZ., Magyar, A. & Oláh, I. Origin of follicular dendritic cell in the chicken spleen. Cell Tissue Res 327, 83–92 (2007). https://doi.org/10.1007/s00441-006-0250-0

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