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The distribution of P2X5 purinergic receptors in the enteric nervous system of mouse

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Abstract

The distribution of the P2X5 purinoceptor in the enteric nervous system of the mouse was studied by immunohistochemistry. P2X5 receptor immunoreactivity was widely distributed in myenteric and submucosal plexuses throughout the gastrointestinal tract. In myenteric plexuses, immunoreactivity for the P2X5 receptor was observed in nerve fibres that enveloped ganglion cell bodies, and possibly on glial cell processes. P2X5 receptor immunoreactivity was colocalised with vasoactive intestinal peptide and surrounded ganglion cells that contained calretinin, calbindin or nitric oxide synthase. In the submucous plexus, P2X5 receptor immunoreactivity occurred throughout the cytoplasm and on the surface membranes of the nerve cells. Double-labelling studies showed that 22%, 9%, 6% and 68% of P2X5 receptor-immunoreactive neurones were also immunoreactive for calretinin, calbindin, nitric oxide synthase and vasoactive intestinal peptide, respectively. Thus, the P2X5 receptor subunit is expressed in specific functional groups of neurones. P2X2 and P2X3 receptors were also present in the mouse enteric plexuses but no immunoreactivity for P2X1, P2X4 or P2X6 receptors was found.

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Acknowledgements

The authors thank Dr. Chrystalla Orphanides for editorial assistance.

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Authors and Affiliations

  1. Department of Neurobiology, Third Military Medical University, Chongqing, 400038, China

    Huai Zhen Ruan

  2. Autonomic Neuroscience Institute, Royal Free & University College Medical School, Rowland Hill Street, London, NW3 2PF, UK

    Huai Zhen Ruan & Geoffrey Burnstock

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  1. Huai Zhen Ruan
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Correspondence to Geoffrey Burnstock.

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Ruan, H.Z., Burnstock, G. The distribution of P2X5 purinergic receptors in the enteric nervous system of mouse. Cell Tissue Res 319, 191–200 (2005). https://doi.org/10.1007/s00441-004-1002-7

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