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DNA fragmentation and detachment of enterocytes induced by anti-CD3 mAb-activated intraepithelial lymphocytes

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Abstract

To elucidate the role of intraepithelial lymphocytes (IEL) and enterocytes in the defense mechanism of the small intestine, we designed experiments to stimulate the IEL by anti-CD3ε, anti-TCRαβ, or anti-TCRγδ monoclonal antibodies (mAbs), and to examine the subsequent changes to the enterocytes. The enterocytes of the duodenum and jejunum, but not of the ileum, showed massive DNA fragmentation 30 min after intraperitoneal injection of anti-CD3 mAb. These responses were also induced by anti-TCRγδ mAb, but not by anti-TCRαβ mAb, and were completely inhibited by cyclosporin A. Nearly half of the enterocytes of the villi in the duodenum and jejunum were exfoliated into the lumen 4 h after the injection of the mAb. Administration of anti-CD3 mAb also induced DNA fragmentation in Fas-deficient MRL/lpr mice, indicating that the Fas-Fas ligand system was not involved in these events. The anti-CD3 mAb treatment also induced massive DNA fragmentation in the intestinal epithelium of the duodenum and jejunum in TNF-receptor-1-deficient mice, whereas TNF-α induced only the detachment of intestinal epithelium of wild-type mice, implying the dissociation of two independent factors and/or mechanisms for DNA fragmentation and the subsequent epithelial cell detachment in the murine duodenum and jejunum. The mAb failed to exfoliate the epithelium in TNF-R1-deficient mice. Thus, TCRγδ+ IEL, when treated with anti-CD3 or anti-TCRγδ mAbs, induced rapid DNA fragmentation and subsequent detachment of the duodenal and jejunal epithelia, but not in the ileum (“the silent ileum”), partly because of the paucity of TCRγδ+ IELs in the ileum.

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Acknowledgements

We express our deepest gratitude to Dr. R. Suzuki, Shionogi Pharmaceutical Company, for his encouragement and helpful discussions throughout the study. We greatly appreciate the expert technical assistance of Mr. M. Ito and Mr. Y. Suzuki. We also thank Ms. J. Shoji and Ms. K. Omori for their secretarial assistance, and Mr. D. Arakawa for reviewing the manuscript.

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Authors and Affiliations

  1. Division of Immunology and Embryology, Department of Cell Biology, Tohoku University School of Medicine, 980-8575, Sendai, Japan

    Kei Yaguchi, Shoichi Kayaba, Hiroyuki Soga, Mai Yamagishi, Shigeru Kasahara & Tsunetoshi Itoh

  2. Third Department of Internal Medicine, Tohoku University School of Medicine, 980-8575, Sendai, Japan

    Shoichi Kayaba, Shuichi Ohara & Takayoshi Toyota

  3. Division of Molecular Metabolism and Diabetes, Tohoku University School of Medicine, 980-8575, Sendai, Japan

    Akira Tamura, Jo Satoh & Yoshitomo Oka

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  1. Kei Yaguchi
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Correspondence to Tsunetoshi Itoh.

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K. Yaguchi and S. Kayaba contributed equally to this work

This work was in part supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture, Japan (07407066, 10470002, and 13670002 to T.I., and 10770001 to H.S.), and by The Funds for Comprehensive Research on Long Term Chronic Diseases from the Ministry of Health and Welfare of Japan (to T.I.)

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Yaguchi, K., Kayaba, S., Soga, H. et al. DNA fragmentation and detachment of enterocytes induced by anti-CD3 mAb-activated intraepithelial lymphocytes. Cell Tissue Res 315, 71–84 (2004). https://doi.org/10.1007/s00441-003-0795-0

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