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Association between TNFRSF11B gene polymorphisms and history of ischemic stroke in Italian diabetic patients

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Abstract

Osteoprotegerin (OPG) is a secretory glycoprotein that belongs to the tumor necrosis factor receptor family and plays a role in atherosclerosis. The present study aimed to evaluate whether OPG gene (TNFRSF11B) polymorphisms are involved in ischemic stroke in an Italian population with diabetes. Participants in a retrospective case–control study included 364 diabetic patients (180 males, 184 females) with history of ischemic stroke and 492 diabetic subjects without history of ischemic stroke (252 males, 240 females). The T245G, T950C, and G1181C polymorphisms of the OPG gene were analyzed by polymerase chain reaction and restriction fragment length polymorphism. We found that the T245G, T950C, and G1181C gene polymorphisms of the OPG gene were significantly (34.1 vs. 9.5 %, P < 0.0001; 30.8 vs. 6.3 %, P < 0.0001 and 26.4 vs. 11.6 % P < 0.0001, respectively) and independently (adjusted OR 5.15 [3.46–7.68], OR 6.63 [4.26–10.31], and OR 3.03 [2.04–4.50], respectively) associated with history of ischemic stroke. We also found that these three polymorphisms act synergistically in patients with stroke history. The TNFRSF11B gene polymorphisms studied are associated with history of ischemic stroke and synergistic effects between these genotypes might be potential markers for cerebrovascular disorders.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Medicine, A. Gemelli University Hospital, Catholic University School of Medicine, Rome, Italy

    Federico Biscetti, Carlo Filippo Porreca, Giovanni Pecorini, Giovanni Ghirlanda & Andrea Flex

  2. Laboratory of Vascular Biology and Genetics, Catholic University School of Medicine, Rome, Italy

    Federico Biscetti, Flavia Angelini, Carlo Filippo Porreca, Giovanni Pecorini, Giovanni Ghirlanda & Andrea Flex

  3. Vascular Medicine and Atherothrombosis Laboratory, Department of Experimental Medicine, Sapienza University of Rome, Polo Pontino, Italy

    Giuseppe Straface

  4. Department of Gerontology and Geriatrics, A. Gemelli University Hospital, Catholic University School of Medicine, Rome, Italy

    Silvia Giovannini

  5. Department of Internal Medicine, Complesso Integrato Columbus, School of Medicine, Rome, Italy

    Angelo Santoliquido & Luca Santoro

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  1. Federico Biscetti
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  2. Giuseppe Straface
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  3. Silvia Giovannini
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  4. Angelo Santoliquido
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  5. Flavia Angelini
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  6. Luca Santoro
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  7. Carlo Filippo Porreca
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  9. Giovanni Ghirlanda
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  10. Andrea Flex
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Correspondence to Andrea Flex.

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Biscetti, F., Straface, G., Giovannini, S. et al. Association between TNFRSF11B gene polymorphisms and history of ischemic stroke in Italian diabetic patients. Hum Genet 132, 49–55 (2013). https://doi.org/10.1007/s00439-012-1224-9

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  • DOI: https://doi.org/10.1007/s00439-012-1224-9

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