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A large open reading frame (orf1995 ) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein

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Abstract

An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.

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Authors and Affiliations

  1. Département de Biochimie, Faculté des sciences et de génie, Université Laval, Québec G1K 7P4, Canada, , , , , , CA

    E. Boudreau & M. Turmel

  2. Département de Biologie Moléculaire, Université de Genève, CH-1211, Genève 4, Switzerland, , , , , , CH

    M. Goldschmidt-Clermont & J.-D. Rochaix

  3. The Doris and Bertie Black Center of Bioenergetics in Life Sciences, Ben Gurion University of the Negev, P.O. Box 653, Beer Sheva, Israel, , , , , , IL

    S. Sivan, A. Michaels & S. Leu

Authors
  1. E. Boudreau
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  2. M. Turmel
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  3. M. Goldschmidt-Clermont
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  4. J.-D. Rochaix
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  5. S. Sivan
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  6. A. Michaels
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  7. S. Leu
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Received: 18 August 1996 / Accepted: 24 September 1996

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Boudreau, E., Turmel, M., Goldschmidt-Clermont, M. et al. A large open reading frame (orf1995 ) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein. Mol Gen Genet 253, 649–653 (1997). https://doi.org/10.1007/s004380050368

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  • DOI: https://doi.org/10.1007/s004380050368

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