Abstract
A simple and sensitive method for linkage analysis is described, which is based on conformation-sensitive gel electrophoresis (CSGE). Using urea-containing agarose gels or a commercially available polyacrylamide-derived matrix, 13 polymorphic markers were newly identified for known genes of the silkworm, Bombyx mori, which had been scored as monomorphic by PCR-RFLP analysis. This method for detecting polymorphisms is quite sensitive, and can be performed with inexpensive reagents and apparatus that is available in most molecular biology laboratories.
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Received: 19 November 1998 / Accepted: 2 March 1999
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Yasukochi, Y. A simple and accurate method for generating co-dominant markers: an application of conformation-sensitive gel electrophoresis to linkage analysis in the silkworm. Mol Gen Genet 261, 796–802 (1999). https://doi.org/10.1007/s004380050023
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DOI: https://doi.org/10.1007/s004380050023