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Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

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Abstract

On-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. Genome-wide gene expression changes in ethylene-treated fruit calyx abscission zone (AZ-C) of Citrus sinensis cv. Olinda were therefore investigated using a citrus genome array representing up to 33,879 citrus transcripts. In total, 1313 and 1044 differentially regulated genes were identified in AZ-C treated with ethylene for 4 and 24 h, respectively. The results showed that mature citrus fruit abscission commenced with the activation of ethylene signal transduction pathway that led to the activation of ethylene responsive transcription factors and the subsequent transcriptional regulation of a large set of ethylene responsive genes. Significantly down-regulated genes included those of starch/sugar biosynthesis, transportation of water and growth promoting hormone synthesis and signaling, whereas significantly up-regulated genes were those involved in defense, cell wall degradation, and secondary metabolism. Our data unraveled the underlying mechanisms of some known important biochemical events occurring at AZ-C and should provide informative suggestions for future manipulation of the events to achieve a controllable abscission for mature citrus fruit.

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Acknowledgments

This work was supported by the International Science and Technology Cooperation Program of China (2012DFA30610), the Science and Technology Planning Project of Guangdong Province (2012A020200016), and by Chongqing Municipal Science and Technology Commission (2007AA1018).

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    Authors and Affiliations

    1. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences Guangzhou, Guangdong, 510640, People’s Republic of China

      Chunzhen Cheng & Guangyan Zhong

    2. College of Horticulture and Landscape Architecture, Southwest University, Beibei, Chongqing, 400715, People’s Republic of China

      Chunzhen Cheng

    3. School of Geographic and Environmental Sciences, Guizhou Normal University Guizhou, Guiyang, 550001, People’s Republic of China

      Lingyun Zhang

    4. College of Agriculture, Guizhou University Guiyang, Guizhou, 550025, People’s Republic of China

      Xuelian Yang

    5. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization Ministry of Agriculture Guangzhou, Guangdong, 510640, People’s Republic of China

      Chunzhen Cheng & Guangyan Zhong

    6. Key Laboratory of Tropical and Subtropical Fruit tree Researches, Guangdong Province Guangzhou, Guangdong, 510640, People’s Republic of China

      Chunzhen Cheng & Guangyan Zhong

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    1. Chunzhen Cheng
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    Correspondence to Guangyan Zhong.

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    Communicated by S. Hohmann.

    C. Cheng and L. Zhang contributed equally.

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    438_2015_1054_MOESM1_ESM.xls

    Supplementary material 1 (XLS 224 kb) Supplemental data S1 The 1568 differentially expressed genes identified by microarray in this study

    Supplementary material 2 (XLS 485 kb) Supplemental data S2 MapMan analysis results of the DEGs identified in this study

    Supplementary material 3 (XLS 20 kb) Supplemental data S3 qRT-PCR primers used in this study

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    Cheng, C., Zhang, L., Yang, X. et al. Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene. Mol Genet Genomics 290, 1991–2006 (2015). https://doi.org/10.1007/s00438-015-1054-2

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