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A novel tomato mutant, Solanum lycopersicum elongated fruit1 (Slelf1), exhibits an elongated fruit shape caused by increased cell layers in the proximal region of the ovary

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Abstract

Genes controlling fruit morphology offer important insights into patterns and mechanisms determining organ shape and size. In cultivated tomato (Solanum lycopersicum L.), a variety of fruit shapes are displayed, including round-, bell pepper-, pear-, and elongate-shaped forms. In this study, we characterized a tomato mutant possessing elongated fruit morphology by histologically analyzing its fruit structure and genetically analyzing and mapping the genetic locus. The mutant line, Solanum lycopersicum elongated fruit 1 (Slelf1), was selected in a previous study from an ethylmethane sulfonate-mutagenized population generated in the background of Micro-Tom, a dwarf and rapid-growth variety. Histological analysis of the Slelf1 mutant revealed dramatically increased elongation of ovary and fruit. Until 6 days before flowering, ovaries were round and they began to elongate afterward. We also determined pericarp thickness and the number of cell layers in three designated fruit regions. We found that mesocarp thickness, as well as the number of cell layers, was increased in the proximal region of immature green fruits, making this the key sector of fruit elongation. Using 262 F2 individuals derived from a cross between Slelf1 and the cultivar Ailsa Craig, we constructed a genetic map, simple sequence repeat (SSR), cleaved amplified polymorphism sequence (CAPS), and derived CAPS (dCAPS) markers and mapped to the 12 tomato chromosomes. Genetic mapping placed the candidate gene locus within a 0.2 Mbp interval on the long arm of chromosome 8 and was likely different from previously known loci affecting fruit shape.

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Abbreviations

AC:

Ailsa Craig

CAPS:

Cleaved amplified polymorphic sequences

DAF:

Days after flowering

DAP:

Days after planting

DBF:

Days before flowering

dCAPs:

Derived cleaved amplified polymorphic sequence

EMS:

Ethylmethane sulfonate

NBRP:

National BioResource Project

Slelf1 :

Solanum lycopersicum elongated fruit 1

SSR markers:

Simple sequence repeat markers

TES markers:

Tomato expressed sequence tag (EST)-derived SSRmarkers

TGS marker:

Tomato genome-derived SSR markers

WT:

Wild type

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Acknowledgments

The authors thank the National BioResearch Project (NBRP), MEXT, Japan, for providing the seed stocks. We also thank all members of Prof. Hiroshi Ezura’s laboratory for helpful discussions throughout the work.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan

    Katarut Chusreeaeom, Tohru Ariizumi, Yoshihiro Okabe & Hiroshi Ezura

  2. Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan

    Erika Asamizu & Kenta Shirasawa

Authors
  1. Katarut Chusreeaeom
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  2. Tohru Ariizumi
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  3. Erika Asamizu
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  4. Yoshihiro Okabe
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  5. Kenta Shirasawa
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  6. Hiroshi Ezura
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Corresponding author

Correspondence to Hiroshi Ezura.

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Communicated by S. Hohmann.

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Chusreeaeom, K., Ariizumi, T., Asamizu, E. et al. A novel tomato mutant, Solanum lycopersicum elongated fruit1 (Slelf1), exhibits an elongated fruit shape caused by increased cell layers in the proximal region of the ovary. Mol Genet Genomics 289, 399–409 (2014). https://doi.org/10.1007/s00438-014-0822-8

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  • DOI: https://doi.org/10.1007/s00438-014-0822-8

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