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Genomic comparison of Salmonella typhimurium DT104 with non-DT104 strains

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Abstract

DT104 emerged as a new branch of Salmonella typhimurium with resistance to multiple antimicrobials. To reveal some general genomic features of DT104 for clues of evolutionary events possibly associated with the emergence of this relatively new type of this pathogen, we mapped 11 independent DT104 strains and compared them with non-DT104 S. typhimurium strains. We found that all 11 DT104 strains contained three insertions absent in non-DT104 strains, i.e., the previously reported ST104, ST104B and ST64B. However, SGI-1, a genomic island known to be responsible for DT104 multidrug resistance, was not present in all DT104 strains examined in this study: one DT104 strain did not contain SGI-1 but carried a 144 kb plasmid, suggesting possible evolutionary relationships between the two DNA elements in the development of antimicrobial resistance.

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Acknowledgments

This work was supported by Genome Canada Grant 256177 to CP; a Grant of National Natural Science Foundation of China (NSFC31100134) to QHZ; a Grant of National Natural Science Foundation of China (NSFC30970078) and a Grant of Natural Science Foundation of Heilongjiang Province of China to GRL; a Heilongjiang Innovation Endowment Award for graduate studies (YJSCX2012-197HLJ) to LT; and Grants of the National Natural Science Foundation of China (NSFC30970119, 81030029, 81271786), a Grant of the National Natural Science Foundation of China and National Institutes of Health of USA (NSFC-NIH 81161120416), and a Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP, 20092307110001) to SLL.

Conflict of interest

All authors have declared that no competing interests exist.

Author information

Author notes

  1. Wei-Qiao Liu

    Present address: Department of Neurology, Vancouver General Hospital, Vancouver, Canada

Authors and Affiliations

  1. Genomics Research Center (one of The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, 157 Baojian Road, 150081, Harbin, China

    Er-Ying Zhao, Hong-Xia Bao, Le Tang, Qing-Hua Zou, Gui-Rong Liu & Shu-Lin Liu

  2. Department of Microbiology, Peking University Health Science Center, Beijing, China

    Qing-Hua Zou & Shu-Lin Liu

  3. Departments of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada

    Wei-Qiao Liu, Jessica Chin, Ying-Ying Dong, Kenneth E. Sanderson & Shu-Lin Liu

  4. Department of Biopharmaceuticals, Faculty of Pharmacy, Harbin Medical University, Harbin, China

    Da-Ling Zhu & Shu-Lin Liu

  5. HMU-UCFM Centre for Infection and Genomics, Harbin Medical University, Harbin, China

    Hong-Xia Bao, Le Tang & Shu-Lin Liu

  6. Genetic Diagnosis Laboratory of First Hospital, Harbin Medical University, Harbin, China

    Yong-Guo Li, Feng-Lin Cao & Shu-Lin Liu

  7. Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, ON, Canada

    Cornelis Poppe

  8. Departments of Biochemistry and Molecular Biology, University of Calgary, Calgary, Canada

    Randal N. Johnston

  9. Department of Biological Sciences, Purdue University, West Lafayette, IN, USA

    Daoguo Zhou

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  1. Er-Ying Zhao
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Corresponding authors

Correspondence to Qing-Hua Zou, Gui-Rong Liu or Shu-Lin Liu.

Additional information

Communicated by D. Ussery.

E.-Y. Zhao, H.-X. Bao, and L. Tang contributed equally to this work.

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Zhao, EY., Bao, HX., Tang, L. et al. Genomic comparison of Salmonella typhimurium DT104 with non-DT104 strains. Mol Genet Genomics 288, 549–557 (2013). https://doi.org/10.1007/s00438-013-0762-8

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