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Transcriptome analysis of the zebrafish mind bomb mutant

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Abstract

Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mib ta52b mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib ta52b mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib ta52b mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib ta52b mutant as compared with the wild-type. Interestingly, it was suggested that the mib ta52b mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.

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Acknowledgments

This study was supported by a Research grant from Ministry of Science and Technology (NBM2300812).

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Authors and Affiliations

  1. School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea

    Junmo Hwang, Hyung-Soo Kim, Ji-Woong Seok, Jun-Dae Kim, Soonil Koun, Jinseok Lee, Hyang Sook Kim, Hyung-Seok Kim, Zae Young Ryoo, Tae-Lin Huh & Sanggyu Lee

  2. Environmental Toxico-Genomic and Proteomic Center, College of Medicine, Korea University, Seoul, South Korea

    So-Young Park

  3. ENH Research Institute, Northwestern University, Evanston, IL, 60201, USA

    San Ming Wang

  4. Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea

    Kil Soo Kim

  5. National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea

    Kyu-Tae Chang

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  1. Junmo Hwang
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  2. Hyung-Soo Kim
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  13. San Ming Wang
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  14. Tae-Lin Huh
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  15. Sanggyu Lee
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Corresponding author

Correspondence to Sanggyu Lee.

Additional information

Communicated by T. Becker.

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438_2008_395_MOESM1_ESM.doc

MOESM1 Confirm the mesoderm-specific expression of zgc:162968 using whole-mount in situ hybridization. Cross-section of the trunk somite shows that zgc:162968 is specifically expressed in somatic mesoderm (arrows). The sectioned level is indicated by black line in left panel. (DOC 1534 kb)

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Hwang, J., Kim, HS., Seok, JW. et al. Transcriptome analysis of the zebrafish mind bomb mutant. Mol Genet Genomics 281, 77–85 (2009). https://doi.org/10.1007/s00438-008-0395-5

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  • DOI: https://doi.org/10.1007/s00438-008-0395-5

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