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Application of physical and genetic map of Rhizobium leguminosarum bv. trifolii TA1 to comparison of three closely related rhizobial genomes

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Abstract

A combined physical and genetic map of Rhizobium leguminosarum biovar trifolii TA1 (RtTA1) genome was constructed and used in comparison of chromosomal organization with the closely related R. leguminosarum bv. viciae 3841 (Rlv) and Rhizobium etli CNF42 (Rhe). This approach allowed evaluation of chromosome and genome plasticity and provided important insights into R. leguminosarum lineage diversity. MssI, SmiI, PacI, and I-CeuI restriction endonucleases were chosen for the analysis, generating fragments with suitable size distributions for RtTA1 genome mapping. The fragments were assembled into a physical map using a combination of complementary methods, including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. About 100 genetic markers were located on the RtTA1 restriction map. Comparison of genetic maps of RtTA1, Rlv, and Rhe revealed extensive chromosomal colinearity despite differences in the physical maps. The comparison provides bases for comprehensive analysis of the evolution of R. leguminosarum genome, indicating that, at least on the chromosomal level, no major rearrangements had occurred after the evolutionary divergence of R. leguminosarum biovars.

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Acknowledgments

Jaroslaw Król and Andrzej Mazur contributed equally to this work. This work was supported by Ministry of Education and Science grant no. 2 P04A 034 26.

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  1. Department of General Microbiology, Institute of Microbiology and Biotechnology, University of Maria Curie Skłodowska, 19 Akademicka St., 20-033, Lublin, Poland

    Jaroslaw E. Król, Andrzej Mazur, Malgorzata Marczak & Anna Skorupska

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  1. Jaroslaw E. Król
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  2. Andrzej Mazur
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  4. Anna Skorupska
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Correspondence to Andrzej Mazur.

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Communicated by D. Ussery.

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Król, J.E., Mazur, A., Marczak, M. et al. Application of physical and genetic map of Rhizobium leguminosarum bv. trifolii TA1 to comparison of three closely related rhizobial genomes. Mol Genet Genomics 279, 107–121 (2008). https://doi.org/10.1007/s00438-007-0299-9

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