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Dark-induced mRNA instability involves RNase E/G-type endoribonuclease cleavage at the AU-box and SD sequences in cyanobacteria

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Abstract

Light-responsive gene expression is crucial to photosynthesizing organisms. Here, we studied functions of cis-elements (AU-box and SD sequences) and a trans-acting factor (ribonuclease, RNase) in light-responsive expression in cyanobacteria. The results indicated that AU-rich nucleotides with an AU-box, UAAAUAAA, just upstream from an SD confer instability on the mRNA under darkness. An RNase E/G homologue, Slr1129, of the cyanobacterium Synechocystis sp. strain PCC 6803 was purified and confirmed capable of endoribonucleolytic cleavage at the AU- (or AG)-rich sequences in vitro. The cleavage depends on the primary target sequence and secondary structure of the mRNA. Complementation tests using Escherichia coli rne/rng mutants showed that Slr1129 fulfilled the functions of both the RNase E and RNase G. An analysis of systematic mutations in the AU-box and SD sequences showed that the cis-elements also affect significantly mRNA stability in light-responsive genes. These results strongly suggested that dark-induced mRNA instability involves RNase E/G-type cleavage at the AU-box and SD sequences in cyanobacteria. The mechanical impact and a possible common mechanism with RNases for light-responsive gene expression are discussed.

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Acknowledgments

This study was supported in part by grants from Scientific Research on Priority Areas from the Ministry of Education, Science, Sports, and Culture of Japan to MA.

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  1. Laboratory of Molecular Genetics, School of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki, 300-0393, Japan

    Yoshinao Horie, Yoko Ito, Miyuki Ono, Naoko Moriwaki, Hideki Kato, Yuriko Hamakubo, Tomoki Amano, Makoto Shirai & Munehiko Asayama

  2. Department of Bioengineering, Tokyo Institute Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8503, Japan

    Masaaki Wachi

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  1. Yoshinao Horie
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Correspondence to Munehiko Asayama.

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Horie, Y., Ito, Y., Ono, M. et al. Dark-induced mRNA instability involves RNase E/G-type endoribonuclease cleavage at the AU-box and SD sequences in cyanobacteria. Mol Genet Genomics 278, 331–346 (2007). https://doi.org/10.1007/s00438-007-0254-9

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