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The transcription factor Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression on a global level

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Abstract

Transcriptomic reprogramming is critical to the coordination between growth and cell cycle progression in response to changing extracellular conditions. In Saccharomyces cerevisiae, the transcription factor Gcr1 contributes to this coordination by supporting maximum expression of G1 cyclins in addition to regulating both glucose-induced and glucose-repressed genes. We report here the comprehensive genome-wide expression profiling of gcr1Δ cells. Our data show that reduced expression of ribosomal protein genes in gcr1Δ cells is detectable both 20 min after glucose addition and in steady-state cultures of raffinose-grown cells, showing that this defect is not the result of slow growth or growth on a repressing sugar. However, the large cell phenotype of the gcr1Δ mutant occurs only in the presence of repressing sugars. GCR1 deletion also results in aberrant derepression of numerous glucose repressed loci; glucose-grown gcr1Δ cells actively respire, demonstrating that this global alteration in transcription corresponds to significant changes at the physiological level. These data offer an insight into the coordination of growth and cell division by providing an integrated view of the transcriptomic, phenotypic, and metabolic consequences of GCR1 deletion.

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Acknowledgments

We thank Kristina Clarke, Nicole Thompson and Baobin Kang for outstanding technical support and other members of the Santangelo lab and USM yeast group for helpful suggestions and comments. This work was supported by a National Institutes of Health Grant RR16476 to G.M.S.

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  1. Mississippi Functional Genomics Network, The University of Southern Mississippi, 118 College Dr., Hattiesburg, MS, USA

    Kellie E. Barbara, Terry M. Haley, Kristine A. Willis & George M. Santangelo

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  1. Kellie E. Barbara
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  2. Terry M. Haley
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  3. Kristine A. Willis
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  4. George M. Santangelo
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Correspondence to George M. Santangelo.

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Communicated by S. Hohmann.

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Barbara, K.E., Haley, T.M., Willis, K.A. et al. The transcription factor Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression on a global level. Mol Genet Genomics 277, 171–188 (2007). https://doi.org/10.1007/s00438-006-0182-0

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