Abstract
The (non-LTR) LINE and Ty3-gypsy-type LTR retrotransposon populations of three Vicia species that differ in genome size (Vicia faba, Vicia melanops and Vicia sativa) have been characterised. In each species the LINE retrotransposons comprise a complex, very heterogeneous set of sequences, while the Ty3-gypsy elements are much more homogeneous. Copy numbers of all three retrotransposon groups (Ty1-copia, Ty3-gypsy and LINE) in these species have been estimated by random genomic sequencing and Southern hybridisation analysis. The Ty3-gypsy elements are extremely numerous in all species, accounting for 18–35% of their genomes. The Ty1-copia group elements are somewhat less abundant and LINE elements are present in still lower amounts. Collectively, 20–45% of the genomes of these three Vicia species are comprised of retrotransposons. These data show that the three retrotransposon groups have proliferated to different extents in members of the Vicia genus and high proliferation has been associated with homogenisation of the retrotransposon population.
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Acknowledgements
PH was funded by a BBSRC Earmark Studentship. This work was supported in part by Grants 960508 (TEBIODIV), 31502 (TEGERM) and FP6-2002-FOOD-1-506223 (GRAIN LEGUMES) from the European Commission under the Frameworks IV, V and VI. We thank Jeff Bennetzen for providing Ty3-gypsy group retrotransposon sequences prior to database submission, Steve Pearce for much helpful advice with the work, Gavin Ramsay for interesting discussions on Vicia phylogeny and for Vicia seeds, and Noel Ellis, Alan Schulman, Amar Kumar, Robbie Waugh and David Marshall for many interesting discussions.
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Communicated by M.-A. Grandbastien
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Hill, P., Burford, D., Martin, D.M. et al. Retrotransposon populations of Vicia species with varying genome size. Mol Genet Genomics 273, 371–381 (2005). https://doi.org/10.1007/s00438-005-1141-x
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DOI: https://doi.org/10.1007/s00438-005-1141-x