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The 5′-leader sequence of sugar beet mitochondrial atp6 encodes a novel polypeptide that is characteristic of Owen cytoplasmic male sterility

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Abstract

Cytoplasmic male sterility (CMS) is a mitochondrially encoded trait, which is characterized by a failure of plants to produce viable pollen. We have investigated the protein profile of mitochondria from sugar beet plants with normal (fertile) or CMS cytoplasm, and observed that a 35-kDa polypeptide is expressed in Owen CMS plants but not in normal plants. The variant 35-kDa polypeptide was found in CMS mitochondria placed in five different nuclear backgrounds. Interestingly, this polypeptide proved to be antigenically related to a 387-codon ORF (preSatp6) that is fused in-frame with the downstream atp6. The presequence extension of the atp6 ORF is commonly found in higher plants, but whether or not it is normally expressed has hitherto remained unclear. Our study is thus the first to demonstrate that the atp6 presequence is actually translated in mitochondria. We also observed that preSATP6 is a mitochondrial membrane protein that assembles into a homogeneous 200-kDa protein complex. In organello translation experiments in the presence of protease inhibitors showed a reduction in the abundance of mature preSATP6 with time, suggesting that the mature preSATP6 may be derived by proteolytic processing of a translation product of the preSatp6/Satp6 ORF.

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Acknowledgements

We gratefully acknowledge Dr. H. Kimura for technical advice and valuable suggestions. This work was done in part at the Research Center for Molecular Genetics, Hokkaido University, and was supported in part by Grants in Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and a Grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences, Japan. M. P. Y. was the recipient of a JSPS Fellowship for Japanese Junior Scientists.

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  1. Masayuki P. Yamamoto

    Present address: National Institute of Agrobiological Sciences, 2-2-1 Kan-nondai, Tsukuba, 305-8602, Japan

Authors and Affiliations

  1. Laboratory of Genetic Engineering, Graduate School of Agriculture, Hokkaido University, N-9, W-9, Kita-ku, Sapporo, 060-8589, Japan

    Masayuki P. Yamamoto, Tomohiko Kubo & Tetsuo Mikami

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  1. Masayuki P. Yamamoto
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  2. Tomohiko Kubo
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  3. Tetsuo Mikami
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Correspondence to Tetsuo Mikami.

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Yamamoto, M.P., Kubo, T. & Mikami, T. The 5′-leader sequence of sugar beet mitochondrial atp6 encodes a novel polypeptide that is characteristic of Owen cytoplasmic male sterility. Mol Genet Genomics 273, 342–349 (2005). https://doi.org/10.1007/s00438-005-1140-y

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