A reverse genetic approach for generating gene replacement mutants in Ustilago maydis

Mol Genet Genomics (2004) DOI 10.1007/s00438-004-1047-z

The plasmid backbone of plasmid pMF1 in Fig.1B was inadvertently depicted the wrong way round. The corrected version is shown below.

Fig. 1

B Schematic representation of the corresponding plasmid system. The optional gfp reporter gene cassette is depicted on the left. T _nos indicates the transcriptional terminator of the nopaline synthase gene from Agrobacterium tumefaciens (Bevan et al. 1983). In the center, mandatory resistance cassettes conferring resistance to carboxin (Cbx^R), hygromycin (Hyg^R), nourseothricin (Nat^R) or phleomycin (Phleo^R) are shown. The optional promoter cassettes derived from the arabinase gene (P _crg1 ), the nitrate reductase gene (P _nar1 ) and the translation elongation factor gene (P _o2tef ) are indicated on the right. The linker sequence between the two Sfi I sites and the plasmid structure is depicted (lacZ’, α fragment of β-galactosidase; Ap^R, Ampicillin resistance; oriC, origin of replication) at the bottom