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Genomic sequence of a 320-kb segment of the Z chromosome of Bombyx mori containing a kettin ortholog

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Abstract

The sex chromosome constitution of the silkworm, Bombyx mori, is ZW in the female and ZZ in the male. Very little molecular information is available about the Z chromosome in Lepidoptera, although the topic is interesting because of the absence of gene dosage compensation in this chromosome. We constructed a 320-kb BAC contig around the Bmkettin gene on the Z chromosome in Bombyx and determined its nucleotide sequence by the shotgun method. We found 13 novel protein-coding sequences in addition to Bmkettin. All the transposable elements detected in the region were truncated, and no LTR retrotransposons were found, in stark contrast to the situation on the W chromosome. In this 320-kb region, four genes for muscle proteins (Bmkettin, Bmtitin1, Bmtitin2, and Bmprojectin) are clustered, together with another gene (Bmmiple) on the Z chromosome in B. mori; their orthologs are also closely linked on chromosome 3 in Drosophila, suggesting a partial synteny. Real-time RT-PCR experiments demonstrated that transcripts of 13 genes of the 14 Z-linked genes found accumulated in larger amounts in males than in female moths, indicating the absence of gene dosage compensation. The implications of these findings for the evolution and function of the Z chromosome in Lepidoptera are discussed.

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Acknowledgements

This work was supported by the Research for the Future Program, JSPS-MEXT (to T.S.), the Basic Research Program, BRAIN (to K.M. and T.S.), and the Animal Genome Research Program, MAFF (to K.M. and T.S.). We are grateful to Prof. M. Kobayashi for his continuous encouragement. We thank J. J. Catanese and B. Swiatkiewicz for helping with BAC library construction, and Y. Ishihara, J. Nohata, I. Matsumoto, M. Takahashi and S. Sasanuma for help with the shotgun sequencing

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  1. M. G. Suzuki

    Present address: Laboratory of Molecular Entomology and Baculovirology, Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, 351-0198, Wako, Saitama, Japan

Authors and Affiliations

  1. Department of Agricultural and Environmental Biology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan

    Y. Koike, M. G. Suzuki & T. Shimada

  2. Genome Research Department, National Institute of Agrobiological Sciences, Owashi 1-2, 305-8643, Tsukuba, Ibaraki, Japan

    K. Mita

  3. Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, 351-0198, Wako, Saitama, Japan

    S. Maeda

  4. Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, 183-8509, Fuchu, Tokyo, Japan

    H. Abe

  5. Children's Hospital Oakland Research Institute, 747 52nd Street, Oakland, CA 94609, USA

    K. Osoegawa & P. J. deJong

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  1. Y. Koike
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S. Maeda: Deceased

Communicated by G. Reuter

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Koike, Y., Mita, K., Suzuki, M.G. et al. Genomic sequence of a 320-kb segment of the Z chromosome of Bombyx mori containing a kettin ortholog. Mol Gen Genomics 269, 137–149 (2003). https://doi.org/10.1007/s00438-003-0822-6

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