Abstract
Trypanosoma cruzi survives in vertebrate and invertebrate hosts and has developed mechanisms that allow it to adapt to changes in the microenvironment such as temperature, pH, and ionic composition. Most of its calcium is concentrated in an organelle named the acidocalcisome, which is acidified by a (V-H+)-adenosine triphosphatase and has H+/Ca2+ countertransportation for calcium uptake. In this work, acidocalcisomes were examined using different transmission electron microscopy techniques. In thin sections of different stages, acidocalcisomes presented a circular shape with an electron-dense inclusion containing P3−, Ca2+, Na+, Mg2+, K+, and Zn2+. They could be distinguished from gold-labeled albumin-containing reservosomes in whole epimastigotes, and a morphometric analysis showed higher amounts of these organelles in amastigotes as compared with epimastigotes and trypomastigotes. It is possible that this variation in the amount of acidocalcisomes in the different evolutive stages could reflect adaptation mechanisms used by the parasite to survive and multiply in different environmental conditions.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 7 September 1999 / Accepted: 17 November 1999
Rights and permissions
About this article
Cite this article
Miranda, K., Benchimol, M., Docampo, R. et al. The fine structure of acidocalcisomes in Trypanosoma cruzi . Parasitol Res 86, 373–384 (2000). https://doi.org/10.1007/s004360050682
Issue Date:
DOI: https://doi.org/10.1007/s004360050682