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Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density-gradient centrifugation

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Abstract

A method for isolation of enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of experimentally infected cats was developed using Percoll density-gradient centrifugation. Gamonts and merozoites were obtained essentially free of host-cell debris. A recovery rate of nearly 30% of the parasites in the original preparations was obtained by this method. Merozoites were separated from gamonts by filtration through a 3-μm polycarbonate filter.

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Authors and Affiliations

  1. Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080, Japan Tel.: 81-155-49-5115 (Ext 5356) Fax: 81-155-49-5359, , , , , , JP

    Yoshitaka Omata, Ayako Taka, Koyuchi Terada, Toshihiro Koyama & Atsushi Saito

  2. Aburahi Laboratories, Shionogi Research Laboratories, Shionogi & Company, Ltd., Kohkacho, Shiga 520-34, Japan, , , , , , JP

    Masanori Kanda

  3. Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Agriculture Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA, , , , , , US

    J. P. Dubey

Authors
  1. Yoshitaka Omata
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  2. Ayako Taka
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  3. Koyuchi Terada
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  4. Toshihiro Koyama
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  5. Masanori Kanda
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  6. Atsushi Saito
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  7. J. P. Dubey
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Additional information

Received: 30 November 1996 / Accepted: 15 January 1997

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Omata, Y., Taka, A., Terada, K. et al. Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density-gradient centrifugation. Parasitol Res 83, 574–577 (1997). https://doi.org/10.1007/s004360050300

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  • DOI: https://doi.org/10.1007/s004360050300

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