Abstract
We describe the expression of SAG-1 cDNA in B6C3F1 mice by microinjecting a 3.3 kbp DNA fragment, consisting of the cytomegalovirus enhancer-chicken β-actin hybrid promoter and SAG-1 into the pronucleus of a fertilized egg at the one-cell stage. Offspring derived from this microinjection were analyzed for the integration and functional expression of the SAG-1 transgene. Steady-state expressions of both the mRNA for SAG-1 and SAG-1 protein product were detected in the brain, thymus, spleen and liver. Approximately 50% of F1 and F2 progeny inherited the SAG-1 transgene from SAG-1 transgenic mice in Mendelian fashion. These results indicated that SAG-1 transgenic lines were established. Transgenic mice harboring the SAG-1 gene will contribute a critical tool of defining the molecular mechanisms of SAG-1 in pathogenesis and host immune response.
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Received: 28 September 1999 / Accepted: 14 October 1999
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Seng, S., Yokoyama, M., Suzuki, R. et al. Expression of SAG-1 of Toxoplasma gondii in transgenic mice. Parasitol Res 86, 263–269 (2000). https://doi.org/10.1007/s004360050041
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DOI: https://doi.org/10.1007/s004360050041