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Effect of Ancylostoma ceylanicum hookworm platelet inhibitor on platelet adhesion and peripheral blood mononuclear cell proliferation

  • Helminthology - Original Paper
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Abstract

Ancylostoma ceylanicum is a zoonotic parasitic nematode that can cause iron-deficiency anemia and malnutrition in humans. A. ceylanicum hookworm platelet inhibitor (Ace-HPI) can inhibit platelet aggregation in the host to facilitate blood sucking, but whether it possesses platelet adhesion inhibitory activity or immunomodulatory role is yet unknown. To explore the effect of Ace-HPI on platelet adhesion, we expressed the recombinant protein in two competent cells, BL21 (DE3) and Rosetta-gami2 (DE3), and incubated this protein with canine platelets in a 96-well microplate. Ace-HPI was used to stimulate peripheral blood mononuclear cells (PBMC) in vitro to investigate the effect on PBMC proliferation and cytokine expression. Results showed that Ace-HPI expressed in Rosetta-gami2 (DE3) strain was mostly soluble. The inhibitory effect of this protein on platelet adhesion was relatively weak (7–8%). This protein stimulated the proliferation of PBMC and promoted the expression of Treg and Th2 cytokines, such as IL-10 and IL-13. These results lay a foundation for exploring the role of Ace-HPI in hookworm disease pathogenesis and as a candidate molecule for hookworm vaccines.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (Grant No. 31672541) and the Science and Technology Planning Project of Guangdong Province, China (Grant No. 2014A020214005).

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Authors and Affiliations

  1. Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China

    Yue Huang, Asmaa M.I. Abuzeid, Tingting Zhuang, Shilan Zhu, Long He, Yunqiu Liu, Qi Zhao, Xiaoyu Chen & Guoqing Li

Authors
  1. Yue Huang
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  2. Asmaa M.I. Abuzeid
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  3. Tingting Zhuang
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  4. Shilan Zhu
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  5. Long He
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  6. Yunqiu Liu
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  7. Qi Zhao
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  8. Xiaoyu Chen
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  9. Guoqing Li
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Correspondence to Guoqing Li.

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The authors declare that they have no conflict of interest.

Ethical approval

All animal experiments followed the guidelines of the Animal Welfare Law and Regulations of the Department of Health and Human Services, China, and were reviewed and approved by the Animal Care and Use Committee of South China Agricultural University.

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Section Editor: Xing-Quan ZHU

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Huang, Y., Abuzeid, A.M., Zhuang, T. et al. Effect of Ancylostoma ceylanicum hookworm platelet inhibitor on platelet adhesion and peripheral blood mononuclear cell proliferation. Parasitol Res 119, 1777–1784 (2020). https://doi.org/10.1007/s00436-020-06678-4

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  • DOI: https://doi.org/10.1007/s00436-020-06678-4

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