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Cryptic species Anopheles daciae (Diptera: Culicidae) found in the Czech Republic and Slovakia

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Abstract

We report the distribution of mosquitoes of the maculipennis complex in two distinct areas of the Czech Republic (Bohemia and South Moravia) and in one locality of neighbouring Slovakia with emphasis on the detection of the newly described cryptic species Anopheles daciae (Linton, Nicolescu & Harbach, 2004). A total of 691 mosquitoes were analysed using a species-specific multiplex PCR assay to differentiate between the members of the maculipennis complex. In the Czech Republic, we found Anopheles maculipennis (with a prevalence rate of 1.4%), Anopheles messeae (49.0%) and Anopheles daciae (49.6%). In Slovakia, only An. messeae (52.1%) and An. daciae (47.9%) were detected. In this study, An. daciae was documented for the first time in the two countries where it represented a markedly higher proportion of maculipennis complex species (with an overall prevalence almost reaching 50%) in comparison to previous reports from Germany, Romania and Poland. The determination of the differential distribution of maculipennis complex species will contribute to assessing risks of mosquito-borne diseases such as malaria or dirofilariasis.

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Acknowledgements

The authors would like to thank Dr. Ivan Gelbič and RNDr. Eduard Stloukal, PhD, for their assistance in mosquito trapping.

Funding

We are grateful to Academy of Sciences of the Czech Republic for financial support (project R200931601) and to Ministry of Health of the Czech Republic - project MH CZ - DRO (NIPH, 75010330).

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Authors and Affiliations

  1. Institute of Vertebrate Biology, v.v.i, Czech Academy of Sciences, Kvetna 8, 603 65, Brno, Czech Republic

    Hana Blažejová, Oldřich Šebesta, Jan Mendel, Lenka Betášová, Juraj Peško, Zdeněk Hubálek & Ivo Rudolf

  2. The National Institute of Public Health, Srobarova 48, Praha 10, 100 42, Czech Republic

    František Rettich

  3. Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovak Republic

    Viktória Čabanová & Martina Miterpáková

  4. Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

    Zdeněk Hubálek & Ivo Rudolf

  5. Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Greifswald, Germany

    Helge Kampen

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  1. Hana Blažejová
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  2. Oldřich Šebesta
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  3. František Rettich
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  4. Jan Mendel
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  5. Viktória Čabanová
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  6. Martina Miterpáková
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  10. Helge Kampen
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Correspondence to Ivo Rudolf.

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Blažejová, H., Šebesta, O., Rettich, F. et al. Cryptic species Anopheles daciae (Diptera: Culicidae) found in the Czech Republic and Slovakia. Parasitol Res 117, 315–321 (2018). https://doi.org/10.1007/s00436-017-5670-0

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  • DOI: https://doi.org/10.1007/s00436-017-5670-0

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