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Occurrence and effect of trematode metacercariae in two endangered killifishes from Greece

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Abstract

We report digeneans (Diplostomidae, Crassiphialinae) in the endangered freshwater fishes Valencia letourneuxi and Valencia robertae, endemics of Western Greece. Digenean metacercariae occurred in two forms in the abdominal cavity, excysted and encysted, the latter attached to the gonads, liver and alimentary tract. Parasites were, using morphological and molecular techniques, identified as two representatives of Crassiphialinae, specifically part of the Posthodiplostomum-Ornithodiplostomum clade. The spatial, seasonal, and age class variation in parasite prevalence was examined. Autumn parasite prevalence varied between the six populations sampled (18.2 to 100%). Seasonal prevalence at the two sites sampled quadannually peaked in autumn and reached its lowest value in spring; prevalence increased with size to 100% in young adult fish. We did not find a correlation between prevalence and host sex. Overall parasites’ weight averaged 0.64% of the host’s, while parasite weight increased with host weight. A comparison of relative condition and hepatosomatic and gonadosomatic indices of infected and metacercariae-free specimens showed that infection did not have a significant effect on host body condition and reproduction. Regarding the parasite’s life cycle, planorbid gastropods are proposed as potential first intermediate hosts in view of the host’s diet and occurrence data of molluscs in the ecosystem. This is the first record of a diplostomid digenean in valenciid fishes and of representatives of the Posthodiplostomum-Ornithodiplostomum clade in a native Greek freshwater fish. Our findings are discussed in conjunction to fish conservation interventions, since parasites may contribute to the decline of endangered species.

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Acknowledgements

This research was conducted in the frame of a wider study on the ecology, biology, genetics, and diet of V. letourneuxi undertaken by scientists of HCMR and was partially funded by the European Union of Aquarium Curators (EUAC) in the frame of the project “Rapid Assessment of the Status of Valencia letourneuxi, the Greek Killifish” (2005-2009). This research received support from the SYNTHESYS Project (http://www.synthesys.info/) (GB-TAF-2984) which is financed by European Community Research Infrastructure Action under the FP7 Integrating Activities Programme. N.K. and M.P.M.V. are supported by the Czech Science Foundation [P505/12/G112 (ECIP)]. The authors wish to thank Andrea Waeschenbach and D. Tim J. Littlewood for the advice regarding the molecular work, Rod A. Bray for the advice regarding parasite identification, the staff of the Wolfson Wellcome Biomedical Laboratories at the Natural History Museum for their technical support, Nicholas Koutsikos for producing the map with the sampling sites’ location, and Marcelo Kovačić for curatorial services. For this study, HCMR had secured all necessary permits for fish collection from the Greek Ministry of Environment, Energy and Climate Change (permit numbers 97429/4350 and 85404/130).

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  1. Institute of Marine Biological Resources and Inland Waters, Hellenic Centre for Marine Research, 46.7 km Athinon – Souniou Av., P.O. Box 712, 190 13, Anavyssos, Greece

    Eleni Kalogianni, Sofia Giakoumi, Yorgos Chatzinikolaou & Maarten P. M. Vanhove

  2. Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    Nikol Kmentová & Maarten P. M. Vanhove

  3. Division of Parasites and Vectors, Department of Life Sciences, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK

    Eileen Harris

  4. Zoological Society of London, Regent’s Park, London, NW1 4RY, UK

    Brian Zimmerman

  5. Laboratory of Biodiversity and Evolutionary Genomics, Department of Biology, University of Leuven, Ch. Deberiotstraat 32, 3000, Leuven, Belgium

    Maarten P. M. Vanhove

  6. Capacities for Biodiversity and Sustainable Development, Operational Directorate Natural Environment, Royal Belgian Institute of Natural Sciences, Vautierstraat 29, 1000, Brussels, Belgium

    Maarten P. M. Vanhove

  7. Centre for Environmental Sciences, Research Group Zoology: Biodiversity and Toxicology, Hasselt University, Agoralaan Gebouw D, 3590, Diepenbeek, Belgium

    Maarten P. M. Vanhove

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  1. Eleni Kalogianni
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Correspondence to Maarten P. M. Vanhove.

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Kalogianni, E., Kmentová, N., Harris, E. et al. Occurrence and effect of trematode metacercariae in two endangered killifishes from Greece. Parasitol Res 116, 3007–3018 (2017). https://doi.org/10.1007/s00436-017-5610-z

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