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Soluble antigen derived from IV larva of Angiostrongylus cantonensis promotes chitinase-like protein 3 (Chil3) expression induced by interleukin-13

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Abstract

Angiostrongyliasis caused by Angiostrongylus cantonensis (A. cantonensis) is an emerging food-borne parasitic disease, which refers basically to eosinophilic meningitis. Chitinase-like protein 3 (Chil3), a member of chitinase-like protein family which has chemotactic activity for eosinophils, is reported to be highly upregulated in brain of mouse infected with A. cantonensis. The mechanisms of high expression of Chil3 and the association between A. cantonensis and Chil3 are rarely reported. In order to understand the mechanism of high expression of Chil3 in A. cantonensis-infected mouse, we measured the level of Chil3 in RAW 264.7 and BV2 cell lines stimulated with soluble antigen of A. cantonensis by qPCR and ELISA. To explore the role of Chil3 in inflammation caused by A. cantonensis, we extracted and cultured brain mononuclear cells (BMNCs) and detected the eosinophil chemotactic activity of Chil3 using transwell assay and flow cytometer. Furthermore, we treated the infected mice by injection with rmChil3 and then counted the number of larvae in brains of infected mice and treated mice to examine the association between the worm and Chil3. Our results showed the soluble antigen from A. cantonensis could promote the Chil3 expression in macrophage and microglial cell lines induced by interleukin-13. In conclusion, we supposed that high expression of Chil3 enhanced by soluble antigens from A. cantonensis might be the reason of serious eosinophil infiltration in mouse brain after A. cantonensis infection.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (NSFC; Nos. 81271855 and 81261160324) to Z-D Wu, NSFC (No. 81572023) to Z-Y Lv, and NSFC (No. 81401686) to J Wei.

Authors’ contributions

FW, JW, XZ, and ZL carried out the experiments and performed the statistical analyses. FW and ZDW drafted the manuscript. ZYL and XZ helped to do the transwell assay section of the study. ZDW and XS conceived the study and coordinated the project. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China

    Feng Wu, Zhen Liu, Xin Zeng, Zilong Yu, Zhiyue Lv, Xi Sun & Zhongdao Wu

  2. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China

    Feng Wu, Jie Wei, Zhen Liu, Xin Zeng, Zilong Yu, Zhiyue Lv, Xi Sun & Zhongdao Wu

  3. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, 510080, China

    Zhongdao Wu

  4. Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China

    Jie Wei

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  1. Feng Wu
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  3. Zhen Liu
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Correspondence to Xi Sun or Zhongdao Wu.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Wu, F., Wei, J., Liu, Z. et al. Soluble antigen derived from IV larva of Angiostrongylus cantonensis promotes chitinase-like protein 3 (Chil3) expression induced by interleukin-13. Parasitol Res 115, 3737–3746 (2016). https://doi.org/10.1007/s00436-016-5135-x

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