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Contribution of Blastocystishominis subtypes and associated inflammatory factors in development of irritable bowel syndrome

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Abstract

Blastocystis hominis with worldwide distribution is a human intestinal protozoa found in all countries. There have been differences in the severity of the pathogenesis of various Blastocystis spp. and a concomitant variation in the plasma concentration of the cytokines in patients with irritable bowel syndrome. In the present study, we aimed to demonstrate the contribution of B. hominis subtypes in the development of irritable bowel syndrome. Stool samples were collected from patients with gastrointestinal disorders. All samples were evaluated through native-lugol method. Total DNA was extracted. A PCR protocol was developed to amplify a specific region of the SSU ribosomal DNA (rDNA) gene. Serum levels of IL-6 and TNF-α were determined by immunoassay methods. The ClustalW algorithm was applied to align and blast the nucleotide sequences of the amplified region of the SSU rDNA gene. To evaluate the phylogenetic and molecular evolutionary of the nucleotide sequences, we used the MEGA software. In this study, we found 26 haplotypes of B. hominis in the studied samples which were collectively belong to five subtypes (ST1, ST2 in patients without irritable bowel syndrome vs. ST3 and two unknown subtypes in patients with irritable bowel syndrome). Result of ELISA showed a high level of IL-6 and TNF-α in the serum of patients with irritable bowel syndrome. The genetic heterogeneity of B. hominis and the existence of different subtypes of the protozoan in patients with IBS may shed light to the fact that some subtypes of parasites may involve in the pathogenesis of IBS.

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Acknowledgments

The work was supported by the Ilam University of Medical Scinences, Ilam, Iran, grant 908441 awarded to Dr. Asad Mirzaei. The authors would like to acknowledge the staff of Dr. Asadollahi laboratory for their kind assistance in the sampling.

Authors’ contributions

MA and BGH were involved in designing and supervising the fieldwork and in interpreting the data; AM, was involved in the collection of samples and molecular tests; MA supervised all genetic aspects in this study. MA and BGH wrote the manuscript. All authors have read and approved the final manuscript. MA is the guarantor of the paper.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran

    Marzieh Azizian, Gholam Basati, Ghobad Abangah & Asad Mirzaei

  2. Department of Biology, Islamic Azad University of Arak, Arak, Iran

    Marzieh Azizian

  3. Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran

    Mohammad Reza Mahmoudi

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  1. Marzieh Azizian
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  2. Gholam Basati
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  3. Ghobad Abangah
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  4. Mohammad Reza Mahmoudi
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  5. Asad Mirzaei
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Correspondence to Asad Mirzaei.

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The study was approved by the Ilam University of Medical Sciences Ethics Committee and registered with no: EC/92/H/132.

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The authors declare that they have no competing interests.

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Azizian, M., Basati, G., Abangah, G. et al. Contribution of Blastocystishominis subtypes and associated inflammatory factors in development of irritable bowel syndrome. Parasitol Res 115, 2003–2009 (2016). https://doi.org/10.1007/s00436-016-4942-4

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  • DOI: https://doi.org/10.1007/s00436-016-4942-4

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